Postmeiotic Sex Chromatin in the Male Germline of Mice

Satoshi H. Namekawa, Peter J. Park, Li Feng Zhang, James E. Shima, John R. McCarrey, Michael D. Griswold, Jeannie T. Lee

Producción científica: Articlerevisión exhaustiva

325 Citas (Scopus)

Resumen

In mammals, the X and Y chromosomes are subject to meiotic sex chromosome inactivation (MSCI) during prophase I in the male germline, but their status thereafter is currently unclear. An abundance of X-linked spermatogenesis genes has spawned the view that the X must be active [1-8]. On the other hand, the idea that the imprinted paternal X of the early embryo may be preinactivated by MSCI suggests that silencing may persist longer [9-12]. To clarify this issue, we establish a comprehensive X-expression profile during mouse spermatogenesis. Here, we discover that the X and Y occupy a novel compartment in the postmeiotic spermatid and adopt a non-Rabl configuration. We demonstrate that this postmeiotic sex chromatin (PMSC) persists throughout spermiogenesis into mature sperm and exhibits epigenetic similarity to the XY body. In the spermatid, 87% of X-linked genes remain suppressed postmeiotically, while autosomes are largely active. We conclude that chromosome-wide X silencing continues from meiosis to the end of spermiogenesis, and we discuss implications for proposed mechanisms of imprinted X-inactivation.

Idioma originalEnglish (US)
Páginas (desde-hasta)660-667
Número de páginas8
PublicaciónCurrent Biology
Volumen16
N.º7
DOI
EstadoPublished - abr 4 2006

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences

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