Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin; Kristina Sikström; Tommy Alain; Masahiro Morita; Shannon McLaughlan; Ola Larsson; Ivan Topisirovic

doi:10.3791/51455

Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin
, Kristina Sikström
, Tommy Alain
, Masahiro Morita
, Shannon McLaughlan
, Ola Larsson
, Ivan Topisirovic

Producción científica: Article › revisión exhaustiva

170 Citas (Scopus)

Resumen

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

Idioma original	English (US)
Publicación	Journal of Visualized Experiments
N.º	87
DOI	https://doi.org/10.3791/51455
Estado	Published - may 17 2014
Publicado de forma externa	Sí

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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AU - McLaughlan, Shannon

AU - Larsson, Ola

AU - Topisirovic, Ivan

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KW - Neoplasms

KW - Nutritional and metabolic diseases

KW - Protein synthesis

KW - Ribosomes

KW - Translatome

KW - eIF4E

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Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

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