Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin, Kristina Sikström, Tommy Alain, Masahiro Morita, Shannon McLaughlan, Ola Larsson, Ivan Topisirovic

Producción científica: Articlerevisión exhaustiva

143 Citas (Scopus)

Resumen

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

Idioma originalEnglish (US)
PublicaciónJournal of Visualized Experiments
N.º87
DOI
EstadoPublished - may 17 2014
Publicado de forma externa

ASJC Scopus subject areas

  • General Chemical Engineering
  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience

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