Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin; Kristina Sikström; Tommy Alain; Masahiro Morita; Shannon McLaughlan; Ola Larsson; Ivan Topisirovic

doi:10.3791/51455

Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin, Kristina Sikström, Tommy Alain, Masahiro Morita, Shannon McLaughlan, Ola Larsson, Ivan Topisirovic

Producción científica: Article › revisión exhaustiva

161 Citas (Scopus)

Resumen

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

Idioma original	English (US)
Publicación	Journal of Visualized Experiments
N.º	87
DOI	https://doi.org/10.3791/51455
Estado	Published - may 17 2014
Publicado de forma externa	Sí

ASJC Scopus subject areas

General Chemical Engineering
General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

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AU - Larsson, Ola

AU - Topisirovic, Ivan

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Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

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