Phosphorylation of the recombinant ρ1 GABA receptor

Anna Sedelnikova, David S. Weiss

Resultado de la investigación: Articlerevisión exhaustiva

9 Citas (Scopus)

Resumen

γ-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain. While a growing body of literature indicates that postsynaptic GABA receptors are regulated by phosphorylation, there is discrepancy as to the specific effects of phosphorylation on GABA receptor function. Here, we have identified phosphorylation sites on the human ρ1 GABA receptor for six protein kinases widely expressed in the brain: Protein kinase C (PKC); cAMP-dependent protein kinase (PKA); calmodulin-dependent kinase (CaMKII); casein kinase (CKII); mitogen-activated protein kinase (MAPK); and cGMP-dependent protein kinase (PKG). We demonstrate that in nearly all cases, the consensus sites and actual phosphorylation sites do not agree supporting the risk of relying on a sequence analysis to identify potential phosphorylation sites. In addition, of the six kinases examined, only CKII phosphorylated the human ρ2 subunit. Site-directed mutagenesis of the phosphorylation sites, or activation/inhibition of select kinase pathways, did not alter the receptor sensitivity or maximal GABA-activated current of the ρ1 GABA receptor expressed in Xenopus laevis oocytes suggesting phosphorylation of ρ1 does not directly alter receptor properties. This study is a first and necessary step towards elucidating the regulation of ρ1 GABA receptors by phosphorylation.

Idioma originalEnglish (US)
Páginas (desde-hasta)237-246
Número de páginas10
PublicaciónInternational Journal of Developmental Neuroscience
Volumen20
N.º3-5
DOI
EstadoPublished - 2002
Publicado de forma externa

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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