Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells

Tracy L. Glass, Timothy D. Raabe, Dana M. García, Joseph R. Koke

Resultado de la investigación: Articlerevisión exhaustiva

11 Citas (Scopus)


Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.

Idioma originalEnglish (US)
Páginas (desde-hasta)43-48
Número de páginas6
PublicaciónBrain Research
EstadoPublished - abr 26 2002
Publicado de forma externa

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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