Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells

Tracy L. Glass; Timothy D. Raabe; Dana M. García; Joseph R. Koke

doi:10.1016/S0006-8993(02)02317-X

Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells

Tracy L. Glass, Timothy D. Raabe, Dana M. García, Joseph R. Koke

Resultado de la investigación: Article › revisión exhaustiva

11 Citas (Scopus)

Resumen

Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.

Idioma original	English (US)
Páginas (desde-hasta)	43-48
Número de páginas	6
Publicación	Brain Research
Volumen	934
N.º	1
DOI	https://doi.org/10.1016/S0006-8993(02)02317-X
Estado	Published - abr 26 2002
Publicado de forma externa	Sí

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells",

abstract = "Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.",

keywords = "Developmental regulation, Neuronal cytoskeleton",

author = "Glass, {Tracy L.} and Raabe, {Timothy D.} and Garc{\'i}a, {Dana M.} and Koke, {Joseph R.}",

note = "Funding Information: This work has been supported in part by grants from the National Science Foundation (NSF DUE #96-50654), Texas Higher Education Coordinating Board (003658-0193-1999), the Faculty Research Enhancement Program and Department of Biology of Southwest Texas State University.",

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T1 - Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells

AU - Glass, Tracy L.

AU - Raabe, Timothy D.

AU - García, Dana M.

AU - Koke, Joseph R.

N1 - Funding Information: This work has been supported in part by grants from the National Science Foundation (NSF DUE #96-50654), Texas Higher Education Coordinating Board (003658-0193-1999), the Faculty Research Enhancement Program and Department of Biology of Southwest Texas State University.

PY - 2002/4/26

Y1 - 2002/4/26

N2 - Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.

AB - Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.

KW - Developmental regulation

KW - Neuronal cytoskeleton

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U2 - 10.1016/S0006-8993(02)02317-X

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AN - SCOPUS:0037177448

SN - 0006-8993

VL - 934

SP - 43

EP - 48

JO - Brain Research

JF - Brain Research

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ER -

Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells

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