Optimization of differentiation of nonhuman primate pluripotent cells using a combinatorial approach

Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, Peter J. Hornsby

Producción científica: Chapter

Resumen

The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination of factors and concentrations is selected in one round of testing, followed by a modification of the combination and subsequent rounds. While this may produce the desired differentiation in the cell population under study, it is also possible that other strategies may be needed to optimize the differentiation process. These strategies are described in this chapter.

Idioma originalEnglish (US)
Título de la publicación alojadaMethods in Molecular Biology
EditorialHumana Press
Páginas187-197
Número de páginas11
DOI
EstadoPublished - 2019

Serie de la publicación

NombreMethods in Molecular Biology
Volumen1919
ISSN (versión impresa)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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