New Screening System for Selection of Anticancer Drugs for Treatment of Human Colorectal Cancer

Werner Scheithauer, Gary M. Clark, Mary P. Moyer, Daniel Von Hoff

Producción científica: Articlerevisión exhaustiva

25 Citas (Scopus)


We report an evaluation of a new radiometric technique (BACTEC assay) as a potential screening system for cytotoxic compounds with activity against cancer of the large bowel. Exponentially growing cells of nine different human colorectal cancer cell lines were exposed to a variety of standard anticancer agents with or without documented clinical activity. Each drug was tested in a series of 1-h and continuous exposure studies utilizing three different concentrations. Antineoplastic effects were analyzed as a function of in vivo achievable serum concentrations, namely by a ratio of the concentration required to decrease cell growth to 10% of control to one-tenth of the peak plasma concentration in humans. Our results suggest that COLO 320DM, OM-1, and Ht-29 cells manifest responsiveness to anticancer drugs consistent with that noted in clinical studies with most agents tested. The radiometric technique provides several advantages for a screening system, including reproducibility, a good agreement with the cloning assay, speed, and low costs. The combined use of the BACTEC technique and the three colon cancer cell lines could prove useful as a screen for new anticancer compounds with activity in colorectal cancer.

Idioma originalEnglish (US)
Páginas (desde-hasta)2703-2708
Número de páginas6
PublicaciónCancer Research
EstadoPublished - jun 1 1986
Publicado de forma externa

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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