New Screening System for Selection of Anticancer Drugs for Treatment of Human Colorectal Cancer

Werner Scheithauer, Gary M. Clark, Mary P. Moyer, Daniel Von Hoff

Producción científica: Articlerevisión exhaustiva

25 Citas (Scopus)

Resumen

We report an evaluation of a new radiometric technique (BACTEC assay) as a potential screening system for cytotoxic compounds with activity against cancer of the large bowel. Exponentially growing cells of nine different human colorectal cancer cell lines were exposed to a variety of standard anticancer agents with or without documented clinical activity. Each drug was tested in a series of 1-h and continuous exposure studies utilizing three different concentrations. Antineoplastic effects were analyzed as a function of in vivo achievable serum concentrations, namely by a ratio of the concentration required to decrease cell growth to 10% of control to one-tenth of the peak plasma concentration in humans. Our results suggest that COLO 320DM, OM-1, and Ht-29 cells manifest responsiveness to anticancer drugs consistent with that noted in clinical studies with most agents tested. The radiometric technique provides several advantages for a screening system, including reproducibility, a good agreement with the cloning assay, speed, and low costs. The combined use of the BACTEC technique and the three colon cancer cell lines could prove useful as a screen for new anticancer compounds with activity in colorectal cancer.

Idioma originalEnglish (US)
Páginas (desde-hasta)2703-2708
Número de páginas6
PublicaciónCancer Research
Volumen46
N.º6
EstadoPublished - jun 1 1986
Publicado de forma externa

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Huella

Profundice en los temas de investigación de 'New Screening System for Selection of Anticancer Drugs for Treatment of Human Colorectal Cancer'. En conjunto forman una huella única.

Citar esto