Myelin basic protein has multiple calmodulin-binding sites

David S. Libich, Christopher M.D. Hill, Jeffery D. Haines, George Harauz

Producción científica: Articlerevisión exhaustiva

31 Citas (Scopus)


Myelin basic protein (MBP) has been shown to bind calmodulin (CaM) in a specific Ca2+-dependent manner via a primary target sequence at its C-terminus [Protein Sci. 12 (2003) 1507]. Upon deimination of MBP, the nature of the interaction changed significantly, suggesting either a new binding site or different conformers with different affinities for CaM. In order to resolve this issue, we investigated here the CaM-binding properties of N- and C-terminal deletion mutants of MBP using Trp fluorescence spectroscopy and mass spectrometry. We conclude that there is an additional CaM-binding site on MBP in a central segment (we posit murine residues 82-93) that forms an amphipathic α-helix.

Idioma originalEnglish (US)
Páginas (desde-hasta)313-319
Número de páginas7
PublicaciónBiochemical and Biophysical Research Communications
EstadoPublished - ago 22 2003
Publicado de forma externa

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry
  • Cell Biology


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