TY - JOUR
T1 - Murine leukemia virus pseudotypes of La Crosse and Hantaan Bunyaviruses
T2 - A system for analysis of cell tropism
AU - Ma, Meihui
AU - Kersten, David B.
AU - Kamrud, Kurt I.
AU - Wool-Lewis, Rouven J.
AU - Schmaljohn, Connie
AU - González-Scarano, Francisco
N1 - Funding Information:
Supported by PHS Grant NS-30606. We thank Paul Bates and Michael Malim (Department of Microbiology, University of Pennsylvania) for their help setting up this system.
PY - 1999/10
Y1 - 1999/10
N2 - We have developed a system for the preparation of La Crosse virus (LAC) and Hantaan virus (HTN) pseudotypes using a murine leukemia virus vector. After concentration, the pseudotypes were present in quantities sufficient to analyze cell tropism and neutralization. Cells resistant to LAC could not be infected with the MLV (LAC) pseudotypes, and the pseudotypes were sensitive to neutralizing monoclonal antibodies prepared against LAC glycoproteins, as well as to inhibition by a soluble form of the virus cell-attachment protein, G1. Perhaps because of lower expression of the HTN glycoproteins at the cell surface, MLV (HTN) pseudotypes were present at lower titers. However, they were also sensitive to appropriate neutralizing antibodies. This pseudotype system will be useful for analysis of the entry process of the Bunyaviridae, and for neutralization studies with some Bunyaviruses whose high virulence normally requires specialized containment facilities. Copyright (C) 1999 Elsevier Science B.V.
AB - We have developed a system for the preparation of La Crosse virus (LAC) and Hantaan virus (HTN) pseudotypes using a murine leukemia virus vector. After concentration, the pseudotypes were present in quantities sufficient to analyze cell tropism and neutralization. Cells resistant to LAC could not be infected with the MLV (LAC) pseudotypes, and the pseudotypes were sensitive to neutralizing monoclonal antibodies prepared against LAC glycoproteins, as well as to inhibition by a soluble form of the virus cell-attachment protein, G1. Perhaps because of lower expression of the HTN glycoproteins at the cell surface, MLV (HTN) pseudotypes were present at lower titers. However, they were also sensitive to appropriate neutralizing antibodies. This pseudotype system will be useful for analysis of the entry process of the Bunyaviridae, and for neutralization studies with some Bunyaviruses whose high virulence normally requires specialized containment facilities. Copyright (C) 1999 Elsevier Science B.V.
KW - Antibodies
KW - Glycoprotein
KW - Hantaan virus
KW - Pseudotype
UR - http://www.scopus.com/inward/record.url?scp=0032840415&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032840415&partnerID=8YFLogxK
U2 - 10.1016/S0168-1702(99)00070-2
DO - 10.1016/S0168-1702(99)00070-2
M3 - Article
C2 - 10500280
AN - SCOPUS:0032840415
SN - 0168-1702
VL - 64
SP - 23
EP - 32
JO - Virus Research
JF - Virus Research
IS - 1
ER -