Mouse amelogenin exons 8 and 9: Sequence analysis and protein distribution

P. Papagerakis, J. M. Ibarra, N. Inozentseva, P. DenBesten, M. MacDougall

Producción científica: Articlerevisión exhaustiva

23 Citas (Scopus)


Amelogenin is the major protein of the developing enamel. Two additional exons, termed 8 and 9, have been characterized in the rat. Our aim was: to identify the mouse amelogenin exons 8/9 sequences; to investigate the potential presence of the alternative spliced isoforms of amelogenin exons 8/9; and to immunolocalize proteins containing sequences encoded by exons 8/9 during odontogenesis. RT-PCR analysis with exon 9 antisense primer generated 2 major amplicons with the use of a mouse tooth cDNA library and dental cell lines. DNA sequence analysis showed 93% identify with the rat exons 8/9 sequence. Alternative splicing of exon 3 was also found, but only in cDNAs lacking exons 8 and 9. Immunohistochemistry localized exons 8/9-encoded proteins in ameloblasts, young odontoblasts, and stratum intermedium cells. Analysis of our data supports the hypothesis that: (1) AMELX contains 2 additional exons; (2) ameloblasts and odontoblasts synthesize amelogenin 8/9; and (3) amelogenin splice variants may have unique functions during tooth formation.

Idioma originalEnglish (US)
Páginas (desde-hasta)613-617
Número de páginas5
PublicaciónJournal of dental research
EstadoPublished - jul 2005

ASJC Scopus subject areas

  • General Dentistry


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