@article{bfea308da78047c99231145122fa1ba7,
title = "Molecular and immunohistochemical analysis of P53 in phaeochromocytoma",
abstract = "We searched for mutations of the p53 gene in 25 phaeochromocytomas using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of the entire conserved region of the gene, encompassing exons 4-8; expression of the p53 protein was assessed by immunohistochemistry. No mutations were found, while a polymorphism in codon 72 was observed. Immunohistochemistry revealed nuclear p53 overexpression in one tumour sample. We conclude that mutations of the {\textquoteleft}hotspot{\textquoteright} region of the p53 gene do not seem to play a role in the pathogenesis of phaeochromocytoma.",
keywords = "Immunohistochemistry, Mokcular screening, Mutation, P53 gene, Phaeochromocytoma, Polymorphism",
author = "Dahia, {P. L.M.} and Aguiar, {R. C.T.} and Tsanaclis, {A. M.} and I. Bendit and Abelin, {N. M.A.} and Toledo, {S. P.A.}",
note = "Funding Information: The authors are indebted to Professor A Grossman for providing the conditions for analysis of exon 4 and for his helpful comments on the manuscript, to Dr SL Chew for critical review of the manuscript and to Drs M Ezabella, C Hayashida. B Mendonga, C Longui, D Malerbi, J Praxedes, H Bernardes and A Pereira for their assistance with some of the tumour specimens. This work was supported in part by Fundaq&o Faculdade de Medicina. CAPES and FAPESP (No. 92 2548-5).",
year = "1995",
month = nov,
doi = "10.1038/bjc.1995.487",
language = "English (US)",
volume = "72",
pages = "1211--1213",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Springer Nature",
number = "5",
}