Modulation by food restriction of intracellular calcium signaling in parotid acinar cells of aging Fischer 344 rats

Jerry G. Olsen; Mohammad A. Salih; Jeff L. Harrison; Irene Herrera; Michael F. Luther; Dike N. Kalu; Meyer D. Lifschitz; Michael S. Katz; Chih Ko Yeh

doi:10.1093/gerona/52A.3.B152

Modulation by food restriction of intracellular calcium signaling in parotid acinar cells of aging Fischer 344 rats

Jerry G. Olsen, Mohammad A. Salih, Jeff L. Harrison, Irene Herrera, Michael F. Luther, Dike N. Kalu, Meyer D. Lifschitz, Michael S. Katz, Chih Ko Yeh

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Resumen

Previous studies suggest that α₁-adrenergic (α₁-AR)-induced intracellular calcium ([Ca²⁺](i)) mobilization in rat parotid acinar cells declines with age. In this study, we examined the effects of food restriction on α₁-AR- and muscarinic-stimulated [Ca²⁺](i) mobilization in parotid acinar cells during aging. [Ca²⁺](i) levels in response to the α₁-AR agonist epinephrine and the muscarinic agonist carbachol were evaluated in Fura-2-loaded parotid acinar cells from ad libitum-fed (AL) and food- restricted (FR) Fischer 344 malt rats at 4, 6, 14, and 24 months of age. [Ca²⁺](i) responses to epinephrine and carbachol (10 μM) were significantly reduced (48% and 35%, respectively; p < .05) in cells from 24- month-old AL rats as compared to younger AL rats. In contrast, no significant reduction of epinephrine and carbachol responses was observed in 24-month- old FR animals. An age-related increase in basal [Ca²⁺](i) (peak around 14 months; p <. 02) was observed in both AL and FR rats. In addition, basal [Ca²⁺](i) was higher in FR than in AL rats at 14 and 24 months of age (p < .02). These studies suggest that FR partially attenuates or delays age- related impairments in α₁-AR- and muscarinic-cholinergic signal transduction systems of parotid acinar cells. Basal [Ca²⁺](i) also appears to be altered during aging and by FR.

Idioma original	English (US)
Páginas (desde-hasta)	B152-B158
Publicación	Journals of Gerontology - Series A Biological Sciences and Medical Sciences
Volumen	52
N.º	3
DOI	https://doi.org/10.1093/gerona/52A.3.B152
Estado	Published - 1997

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General Medicine

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Olsen, J. G., Salih, M. A., Harrison, J. L., Herrera, I., Luther, M. F., Kalu, D. N., Lifschitz, M. D., Katz, M. S., & Yeh, C. K. (1997). Modulation by food restriction of intracellular calcium signaling in parotid acinar cells of aging Fischer 344 rats. Journals of Gerontology - Series A Biological Sciences and Medical Sciences, 52(3), B152-B158. https://doi.org/10.1093/gerona/52A.3.B152

Olsen, JG, Salih, MA, Harrison, JL, Herrera, I, Luther, MF, Kalu, DN, Lifschitz, MD, Katz, MS & Yeh, CK 1997, 'Modulation by food restriction of intracellular calcium signaling in parotid acinar cells of aging Fischer 344 rats', Journals of Gerontology - Series A Biological Sciences and Medical Sciences, vol. 52, n.º 3, pp. B152-B158. https://doi.org/10.1093/gerona/52A.3.B152

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title = "Modulation by food restriction of intracellular calcium signaling in parotid acinar cells of aging Fischer 344 rats",

abstract = "Previous studies suggest that α1-adrenergic (α1-AR)-induced intracellular calcium ([Ca2+](i)) mobilization in rat parotid acinar cells declines with age. In this study, we examined the effects of food restriction on α1-AR- and muscarinic-stimulated [Ca2+](i) mobilization in parotid acinar cells during aging. [Ca2+](i) levels in response to the α1-AR agonist epinephrine and the muscarinic agonist carbachol were evaluated in Fura-2-loaded parotid acinar cells from ad libitum-fed (AL) and food- restricted (FR) Fischer 344 malt rats at 4, 6, 14, and 24 months of age. [Ca2+](i) responses to epinephrine and carbachol (10 μM) were significantly reduced (48% and 35%, respectively; p < .05) in cells from 24- month-old AL rats as compared to younger AL rats. In contrast, no significant reduction of epinephrine and carbachol responses was observed in 24-month- old FR animals. An age-related increase in basal [Ca2+](i) (peak around 14 months; p <. 02) was observed in both AL and FR rats. In addition, basal [Ca2+](i) was higher in FR than in AL rats at 14 and 24 months of age (p < .02). These studies suggest that FR partially attenuates or delays age- related impairments in α1-AR- and muscarinic-cholinergic signal transduction systems of parotid acinar cells. Basal [Ca2+](i) also appears to be altered during aging and by FR.",

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AU - Luther, Michael F.

AU - Kalu, Dike N.

AU - Lifschitz, Meyer D.

AU - Katz, Michael S.

AU - Yeh, Chih Ko

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N2 - Previous studies suggest that α1-adrenergic (α1-AR)-induced intracellular calcium ([Ca2+](i)) mobilization in rat parotid acinar cells declines with age. In this study, we examined the effects of food restriction on α1-AR- and muscarinic-stimulated [Ca2+](i) mobilization in parotid acinar cells during aging. [Ca2+](i) levels in response to the α1-AR agonist epinephrine and the muscarinic agonist carbachol were evaluated in Fura-2-loaded parotid acinar cells from ad libitum-fed (AL) and food- restricted (FR) Fischer 344 malt rats at 4, 6, 14, and 24 months of age. [Ca2+](i) responses to epinephrine and carbachol (10 μM) were significantly reduced (48% and 35%, respectively; p < .05) in cells from 24- month-old AL rats as compared to younger AL rats. In contrast, no significant reduction of epinephrine and carbachol responses was observed in 24-month- old FR animals. An age-related increase in basal [Ca2+](i) (peak around 14 months; p <. 02) was observed in both AL and FR rats. In addition, basal [Ca2+](i) was higher in FR than in AL rats at 14 and 24 months of age (p < .02). These studies suggest that FR partially attenuates or delays age- related impairments in α1-AR- and muscarinic-cholinergic signal transduction systems of parotid acinar cells. Basal [Ca2+](i) also appears to be altered during aging and by FR.

AB - Previous studies suggest that α1-adrenergic (α1-AR)-induced intracellular calcium ([Ca2+](i)) mobilization in rat parotid acinar cells declines with age. In this study, we examined the effects of food restriction on α1-AR- and muscarinic-stimulated [Ca2+](i) mobilization in parotid acinar cells during aging. [Ca2+](i) levels in response to the α1-AR agonist epinephrine and the muscarinic agonist carbachol were evaluated in Fura-2-loaded parotid acinar cells from ad libitum-fed (AL) and food- restricted (FR) Fischer 344 malt rats at 4, 6, 14, and 24 months of age. [Ca2+](i) responses to epinephrine and carbachol (10 μM) were significantly reduced (48% and 35%, respectively; p < .05) in cells from 24- month-old AL rats as compared to younger AL rats. In contrast, no significant reduction of epinephrine and carbachol responses was observed in 24-month- old FR animals. An age-related increase in basal [Ca2+](i) (peak around 14 months; p <. 02) was observed in both AL and FR rats. In addition, basal [Ca2+](i) was higher in FR than in AL rats at 14 and 24 months of age (p < .02). These studies suggest that FR partially attenuates or delays age- related impairments in α1-AR- and muscarinic-cholinergic signal transduction systems of parotid acinar cells. Basal [Ca2+](i) also appears to be altered during aging and by FR.

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Modulation by food restriction of intracellular calcium signaling in parotid acinar cells of aging Fischer 344 rats

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