TY - JOUR
T1 - Modelo de encefalopatia hepática induzida por tioacetamida em camundongos C57BL/6
T2 - Estudo comportamental e neuroquímico
AU - de Miranda, Aline Silva
AU - Rodrigues, David Henrique
AU - Vieira, Luciene Bruno
AU - Lima, Cristiano Xavier
AU - Rachid, Milene Alvarenga
AU - Vidigal, Paula Vieira Teixeira
AU - Gomez, Marcus Vinicius
AU - dos Reis, Helton José
AU - Guatimosim, Cristina
AU - Teixeira, Antônio Lúcio
PY - 2010/8
Y1 - 2010/8
N2 - Objective: Hepatic encephalopathy (HE) is a neuropsychiatric syndrome resulting from liver failure. In the present study, we aimed to standardize an animal model of HE induced by thioacetamide (TAA) in C57BL/6 mice evaluating behavioral symptoms in association with liver damage and alterations in neurotransmitter release. Method: HE was induced by an intraperitoneal single dose of TAA (200 mg/kg, 600 mg/kg or 1,200 mg/kg). Behavioral symptoms were evaluated using the SHIRPA battery. Liver damage was confirmed by histopathological analysis. The glutamate release was measured using fluorimetric assay. Results: The neuropsychiatric state, motor behavior and reflex and sensory functions were significantly altered in the group receiving 600 mg/kg of TAA. Biochemical analysis revealed an increase in the glutamate release in the cerebral cortex of HE mice. Conclusion: HE induced by 600mg/kg TAA injection in C57BL/6 mice seems to be a suitable model to investigate the pathogenesis and clinical disorders of HE.
AB - Objective: Hepatic encephalopathy (HE) is a neuropsychiatric syndrome resulting from liver failure. In the present study, we aimed to standardize an animal model of HE induced by thioacetamide (TAA) in C57BL/6 mice evaluating behavioral symptoms in association with liver damage and alterations in neurotransmitter release. Method: HE was induced by an intraperitoneal single dose of TAA (200 mg/kg, 600 mg/kg or 1,200 mg/kg). Behavioral symptoms were evaluated using the SHIRPA battery. Liver damage was confirmed by histopathological analysis. The glutamate release was measured using fluorimetric assay. Results: The neuropsychiatric state, motor behavior and reflex and sensory functions were significantly altered in the group receiving 600 mg/kg of TAA. Biochemical analysis revealed an increase in the glutamate release in the cerebral cortex of HE mice. Conclusion: HE induced by 600mg/kg TAA injection in C57BL/6 mice seems to be a suitable model to investigate the pathogenesis and clinical disorders of HE.
KW - Behavioral changes
KW - Glutamate
KW - Hepatic encephalopathy
KW - SHIRPA
KW - Thioacetamide
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UR - http://www.scopus.com/inward/citedby.url?scp=77957296799&partnerID=8YFLogxK
U2 - 10.1590/s0004-282x2010000400022
DO - 10.1590/s0004-282x2010000400022
M3 - Article
C2 - 20730316
AN - SCOPUS:77957296799
SN - 0004-282X
VL - 68
SP - 597
EP - 602
JO - Arquivos de neuro-psiquiatria
JF - Arquivos de neuro-psiquiatria
IS - 4
ER -