miR-21: An androgen receptor-regulated microRNA that promotes hormone-dependent and hormone-independent prostate cancer growth

Judit Ribas, Xiaohua Ni, Michael Haffner, Erik A. Wentzel, Amirali Hassanzadeh Salmasi, Wasim H. Chowdhury, Tarana A. Kudrolli, Srinivasan Yegnasubramanian, Jun Luo, Ron Rodriguez, Joshua T. Mendell, Shawn E. Lupold

Producción científica: Articlerevisión exhaustiva

384 Citas (Scopus)

Resumen

Androgen receptor (AR)-mediated oncogenic pathways have not been fully elucidated. In this study, we used high-throughput microarray analysis on two AR-positive prostate cancer (CaP) cell lines to identify 16 AR-responsive microRNAs (miRNA). We focused on miR-21 because of its previously reported oncogenic activity in other cancers. We show androgen-induced AR binding to the defined miR-21 promoter, miPPR-21, suggesting direct transcriptional regulation. Inhibition of miR-21 diminished androgen-induced CaP cell proliferation, providing new evidence that miRNAs can contribute to androgen-driven cell growth. Elevated expression of miR-21 enhanced CaP tumor growth in vivo and, surprisingly, was sufficient for androgen-dependent tumors to overcome castration-mediated growth arrest. Thus, elevated miR-21 expression alone is sufficient to impart castration resistance. Moreover, quantitative reverse transcription-PCR analysis revealed elevated miR-21 expression in CaP when compared with adjacent normal tissue. These results suggest that miR-21 may contribute to CaP pathogenesis.

Idioma originalEnglish (US)
Páginas (desde-hasta)7165-7169
Número de páginas5
PublicaciónCancer Research
Volumen69
N.º18
DOI
EstadoPublished - 2009
Publicado de forma externa

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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