Membrane potential of rat adipocytes: Effect of phospholipase C, concanavalin A, and adenosine

F. L. Kiechle, F. Bailey, N. Hill, T. Malinski

Producción científica: Articlerevisión exhaustiva

2 Citas (Scopus)

Resumen

The change in transmembrane potential of rat adipocytes was measured using the fluorescent probe 3,3'-diethylthiadicarbocyanine iodide, diS-C2-(5). The method was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM (Bailey et al: Bioelectrochem. Bioenergetics 21:333-42. 1989). Two insulin-mimetic agents, phospholipase C from Clostridium perfringens and concanavalin A, induced a dose dependent hyperpolarization of rat epididymal adipocytes, like insulin. Removal of endogenous adenosine with adenosine deaminase or adenosine receptor blockade with isobutylmethylxanthine following the initiation of insulin-induced hyperpolarization resulted in depolarization. These same agents induced hyperpolarization of -6 to -8 mV when added without insulin. The replacement of adenosine with its analogue, N6-phenylisopropyladenosine, plus insulin depolarized the cells toward the transmembrane potential established by insulin, -2.0 mV. These studies suggest that adenosine receptor occupancy is required to maintain insulin- induced hyperpolarization.

Idioma originalEnglish (US)
Páginas (desde-hasta)164-172
Número de páginas9
PublicaciónAnnals of Clinical and Laboratory Science
Volumen24
N.º2
EstadoPublished - 1994
Publicado de forma externa

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology
  • Molecular Biology
  • Hematology
  • Clinical Biochemistry
  • Medical Laboratory Technology

Huella

Profundice en los temas de investigación de 'Membrane potential of rat adipocytes: Effect of phospholipase C, concanavalin A, and adenosine'. En conjunto forman una huella única.

Citar esto