Mediator function of the human Rad51B-Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange

Stefan Sigurdsson, Stephen Van Komen, Wendy Bussen, David Schild, Joanna S. Albala, Patrick Sung

Resultado de la investigación: Articlerevisión exhaustiva

185 Citas (Scopus)

Resumen

Five Rad51-like proteins, referred to as Rad51 paralogs, have been described in vertebrates. We show that two of them, Rad51B and Rad51C, are associated in a stable complex. Rad51B-Rad51C complex has ssDNA binding and ssDNA-stimulated ATPase activities. We also examined the functional interaction of Rad51B-Rad51C with Rad51 and RPA. Even though RPA enhances Rad51-catalyzed DNA joint formation via removal of secondary structure in the ssDNA substrate, it can also compete with Rad51 for binding to the substrate, leading to suppressed reaction efficiency. The competition by RPA for substrate binding can be partially alleviated by Rad51B-Rad51C. This recombination mediator function of Rad51B-Rad51C is likely required for the assembly of the Rad51-ssDNA nucleoprotein filament in vivo.

Idioma originalEnglish (US)
Páginas (desde-hasta)3308-3318
Número de páginas11
PublicaciónGenes and Development
Volumen15
N.º24
DOI
EstadoPublished - dic. 15 2001

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Huella

Profundice en los temas de investigación de 'Mediator function of the human Rad51B-Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange'. En conjunto forman una huella única.

Citar esto