TY - JOUR
T1 - Mapping and Identification of Native Proteins of Developing Teeth in Mouse Mandibles
AU - Colley, Madeline
AU - Liang, Sitai
AU - Tan, Chunyan
AU - Trobough, Kyle P.
AU - Bach, Stephan B.H.
AU - Chun, Yong Hee Patricia
N1 - Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/6/2
Y1 - 2020/6/2
N2 - Mass spectrometry imaging is a powerful tool of increasing utility due to its ability to spatially resolve molecular biomarkers directly from sectioned tissues. One hindrance to its universality is that no single protocol is sufficient for every tissue type, fixation, and pretreatment. Mineralized tissues are uniquely challenging as extensive decalcification protocols are necessary to achieve thin sections. In this study, we optimized a method to image tryptic peptides by matrix-Assisted laser desorption ionization mass spectrometry of decalcified, formalin-fixed paraffin-embedded mouse hemimandibles. Using a combination of on-Tissue MS/MS and hydrogel extraction LC-MS/MS, peptides from the enamel, dentin, periodontal ligament, alveolar bone, pulp, and other regions are identified and mapped. This breakthrough method provides a comprehensive approach to biomarker discovery in dental and craniofacial tissues which is highly relevant given that diseases originating from this region of the body are the most prevalent across all populations.
AB - Mass spectrometry imaging is a powerful tool of increasing utility due to its ability to spatially resolve molecular biomarkers directly from sectioned tissues. One hindrance to its universality is that no single protocol is sufficient for every tissue type, fixation, and pretreatment. Mineralized tissues are uniquely challenging as extensive decalcification protocols are necessary to achieve thin sections. In this study, we optimized a method to image tryptic peptides by matrix-Assisted laser desorption ionization mass spectrometry of decalcified, formalin-fixed paraffin-embedded mouse hemimandibles. Using a combination of on-Tissue MS/MS and hydrogel extraction LC-MS/MS, peptides from the enamel, dentin, periodontal ligament, alveolar bone, pulp, and other regions are identified and mapped. This breakthrough method provides a comprehensive approach to biomarker discovery in dental and craniofacial tissues which is highly relevant given that diseases originating from this region of the body are the most prevalent across all populations.
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U2 - 10.1021/acs.analchem.0c00359
DO - 10.1021/acs.analchem.0c00359
M3 - Article
C2 - 32362116
AN - SCOPUS:85086504444
SN - 0003-2700
VL - 92
SP - 7630
EP - 7637
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 11
ER -