Long-term infuence of bone marrow-derived mesenchymal stem cells on liver ischemia-reperfusion injury in a rat model

Maciej Nowacki, Łukasz Nazarewski, Marta Pokrywczyńska, Tomasz Kloskowski, Dominik Tyloch, Katarzyna Pietkun, Arkadiusz Jundziłł, Marta Rasmus, Karolina Warda, Maciej Gagat, Alina Grzanka, Magdalena Bodnar, Andrzej Marszałek, Marek Krawczyk, Samy L. Habib, Tomasz Drewa

Resultado de la investigación: Articlerevisión exhaustiva

17 Citas (Scopus)


Background: The aim of this study was to evaluate the long-term usefulness of intraportal injection of the bone marrow-derived mesenchymal stem cells (BM-MSCs) in limitation of experimentally induced ischemia-reperfusion injury (IRI) in a rat model. Material/Methods: Twenty Wistar rats were divided into 3 groups: donor group (n=5), study group (n=10), and control group (n=5). IRI was performed using a modifed hanging-weight system after left portal triad occlusion in study group animals. Isolated autologous BM-MSCs were labeled with fuorochrome PKH-26 then intraportally injected into the rats in the study group. Control group animals were intraportally injected with 1 ml of PBS. Follow-up was 3 months, after which animals were sacrifced for histopathological examination. Migration of BM-MSCs into diferent organs was examined. Results: H&E staining of liver tissue sections from “time zero” biopsies did not show many irregularities in structural or histological construction compared to liver sections from the control group. However, a small amount of cen-trilobular hepatocyte necrosis and coagulative necrosis with neutrophil infltration areas was observed in liver sections of the study group. The migration assay of BM-MSCs labeled with PKH-26 showed the highest positive BM-MSCs staining (6%) in the spleen, while few positively stained cells were found (2%) in liver sections. No BM-MSCs were detected in brain, kidney, or lung tissues. Conclusions: These results suggest that intraportal bone marrow-de rived mesenchymal stem cell injection is safe and cells do not migrate chaotically to other organs after targeted implementation.

Idioma originalEnglish (US)
Páginas (desde-hasta)132-140
Número de páginas9
PublicaciónAnnals of Transplantation
EstadoPublished - mar 10 2015

ASJC Scopus subject areas

  • Transplantation


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