Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)