Lentiviral infection of mouse bone marrow cells for hematopoietic stem cell transplantation

Cang Chen, Michael J. Guderyon, Guo Ge, Robert A. Clark, Senlin Li

Producción científica: Chapter

1 Cita (Scopus)


Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.

Idioma originalEnglish (US)
Título de la publicación alojadaMethods in Molecular Biology
EditorialHumana Press
Número de páginas9
EstadoPublished - 2019

Serie de la publicación

NombreMethods in Molecular Biology
ISSN (versión impresa)1064-3745

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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