TY - JOUR
T1 - Large inverted repeats in the vicinity of a single double-strand break strongly affect repair in yeast diploids lacking Rad51
AU - Downing, Brandon
AU - Morgan, Rachel
AU - VanHulle, Kelly
AU - Deem, Angela
AU - Malkova, Anna
PY - 2008/10/14
Y1 - 2008/10/14
N2 - DNA double-strand breaks (DSBs) are critical lesions that can lead to cell death or chromosomal rearrangements. Rad51 is necessary for most mitotic and meiotic DSB repair events, although a number of RAD51-independent pathways exist. Previously, we described DSB repair in rad51Δ yeast diploids that was stimulated by a DNA region termed "facilitator of break-induced replication" (FBI) located approximately 30 kb from the site of an HO-induced DSB. Here, we demonstrate that FBI is a large inverted DNA repeat that channels the repair of DSBs into the single-strand annealing-gross chromosomal rearrangements (SSA-GCR) pathway. Further, analysis of DSB repair in rad54Δ cells allowed us to propose that the SSA-GCR repair pathway is suppressed in the presence of Rad51p. Therefore, an additional role of Rad51 might be to protect eukaryotic genomes from instabilities by preventing chromosomal rearrangements.
AB - DNA double-strand breaks (DSBs) are critical lesions that can lead to cell death or chromosomal rearrangements. Rad51 is necessary for most mitotic and meiotic DSB repair events, although a number of RAD51-independent pathways exist. Previously, we described DSB repair in rad51Δ yeast diploids that was stimulated by a DNA region termed "facilitator of break-induced replication" (FBI) located approximately 30 kb from the site of an HO-induced DSB. Here, we demonstrate that FBI is a large inverted DNA repeat that channels the repair of DSBs into the single-strand annealing-gross chromosomal rearrangements (SSA-GCR) pathway. Further, analysis of DSB repair in rad54Δ cells allowed us to propose that the SSA-GCR repair pathway is suppressed in the presence of Rad51p. Therefore, an additional role of Rad51 might be to protect eukaryotic genomes from instabilities by preventing chromosomal rearrangements.
KW - Double-strand DNA breaks
KW - Inverted DNA repeats
KW - rad51Δ
KW - rad54Δ
KW - Recombination
KW - Yeast
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U2 - 10.1016/j.mrfmmm.2008.07.013
DO - 10.1016/j.mrfmmm.2008.07.013
M3 - Article
C2 - 18755201
AN - SCOPUS:53349165577
SN - 0027-5107
VL - 645
SP - 9
EP - 18
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1-2
ER -