Kinetics of nitrogen oxide production following experimental thermal injury in rats

William K. Becker, Ronald L. Shippee, Albert T. Mc Manus, Arthur D. Mason, Basil A. Pruitt

Resultado de la investigación: Articlerevisión exhaustiva

11 Citas (Scopus)


Nitric oxide is biosynthesized from the amino acid L-arginine by the enzyme nitric oxide synthase. Nitric oxide is a vasodilator, a neurotransmitter, and may modulate immune function. The experiments presented here were performed to determine whether the synthesis of nitric oxide is increased following experimental burn injury in rats. After a 30% total body surface area burn in 300-g Lewis rats, the urinary output of nitrate, a stable metabolite of nitric oxide, was significantly increased for 8 days postburn compared with that in sham-burned control rats. The origin of the urinary nitrate from L-arginine was demonstrated by administering the stable isotope 15 N 2 -guanido-arginine to burned and sham-burned rats and observing an immediate enrichment of 15 N in nitrate. The amount of administered 15 N recovered as 15 NO 3 was <1% of the administered arginine isotope in both the burned and unburned rats; the recovery of the isotope increased tenfold over baseline recovery in burned rats. The arginine analog N-monomethyl-arginine, an inhibitor of the enzyme nitric oxide synthase, blocked the postburn rise in urinary NO 3 output in burned rats, but did not completely inhibit the output of NO 3 in burn wound-infected rats. Experimental burn injury in rats results in an increase in L-arginine-dependent nitric oxide production and urinary nitrate output.

Idioma originalEnglish (US)
Páginas (desde-hasta)855-862
Número de páginas8
PublicaciónJournal of Trauma - Injury, Infection and Critical Care
EstadoPublished - jun 1993

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine


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