Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

Yu Cong; Madhulika Jupelli; M. Neal Guentzel; Guangming Zhong; Ashlesh K. Murthy; Bernard P. Arulanandam

doi:10.1016/j.vaccine.2007.02.010

Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

Yu Cong, Madhulika Jupelli, M. Neal Guentzel, Guangming Zhong, Ashlesh K. Murthy, Bernard P. Arulanandam

Department of Microbiology, Immunology & Molecular Genetics

Producción científica: Article › revisión exhaustiva

56 Citas (Scopus)

Resumen

We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.

Idioma original	English (US)
Páginas (desde-hasta)	3773-3780
Número de páginas	8
Publicación	Vaccine
Volumen	25
N.º	19
DOI	https://doi.org/10.1016/j.vaccine.2007.02.010
Estado	Published - may 10 2007

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2007.02.010

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title = "Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection",

abstract = "We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.",

keywords = "Adjuvant, CPAF, Chlamydia, Chlamydial protease-like activity factor, CpG, Genital, Vaccine",

author = "Yu Cong and Madhulika Jupelli and Guentzel, \{M. Neal\} and Guangming Zhong and Murthy, \{Ashlesh K.\} and Arulanandam, \{Bernard P.\}",

note = "Funding Information: This work was supported by National Institutes of Health grants AR048973 and GM08194. The authors thank Dr. Jieh-Juen Yu for critical review of the manuscript.",

year = "2007",

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doi = "10.1016/j.vaccine.2007.02.010",

language = "English (US)",

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AU - Cong, Yu

AU - Jupelli, Madhulika

AU - Guentzel, M. Neal

AU - Zhong, Guangming

AU - Murthy, Ashlesh K.

AU - Arulanandam, Bernard P.

N1 - Funding Information: This work was supported by National Institutes of Health grants AR048973 and GM08194. The authors thank Dr. Jieh-Juen Yu for critical review of the manuscript.

PY - 2007/5/10

Y1 - 2007/5/10

N2 - We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.

AB - We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.

KW - Adjuvant

KW - CPAF

KW - Chlamydia

KW - Chlamydial protease-like activity factor

KW - CpG

KW - Genital

KW - Vaccine

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Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

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