Intracellular Na+, K+ and Cl- activities in Ehrlich ascites tumor cells

W. David Dawson, Thomas C. Smith

Resultado de la investigación: Articlerevisión exhaustiva

19 Citas (Scopus)

Resumen

Ehrlich ascites tumor cell membrane potential (Vm) and intracellular Na+, K+ and Cl- activities were measured under steady-state conditions in normal saline medium (Na+ = 154, K+ = 6, Cl- = 150 mequiv./l). Membrane potential was estimated to be -23.3 ± 0.8 mV using glass microelectrodes. Intracellular ion activities were estimated with similar glass electrodes rendered ion-selective by incorporation of ion-specific ionophores. Measurements of Vm and ion-activity differences were made in the same populations of cells. Under these conditions the intracellular Na+, K+ and Cl- activities are 4.6 ± 0.5; 68.3 ± 8.0; and 43.6 ± 2.1 mequiv./l, respectively. The apparent activity coefficients for Na+ and K+ are 0.18 ± 0.02 and 0.41 ± 0.05 respectively. These are significantly lower than the activity coefficients expected for the ions in physiological salt solutions (0.71 and 0.73, respectively). The activity coefficient for intracellular Cl- (0.67 ± 0.03), however, is close to that of the medium (0.73), and the transmembrane electrochemical potential difference for Cl- is not different from zero. The results establish that the energy available from the Na+ electrochemical gradient is much greater than previously estimated from chemical measurements.

Idioma originalEnglish (US)
Páginas (desde-hasta)293-300
Número de páginas8
PublicaciónBBA - Biomembranes
Volumen860
N.º2
DOI
EstadoPublished - ago. 21 1986
Publicado de forma externa

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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