Interleukin 15 skews monocyte differentiation into dendritic cells with features of langerhans cells

M. Mohamadzadeh, F. Berard, G. Essert, C. Chalouni, B. Pulendran, J. Davoust, G. Bridges, A. K. Palucka, J. Banchereau

Producción científica: Articlerevisión exhaustiva

239 Citas (Scopus)

Resumen

Langerhans cells (LCs) represent a subset of immature dendritic cells (DCs) specifically localized in the epidermis and other mucosal epithelia. As surrounding keratinocytes can produce interleukin (IL)-15, a cytokine that utilizes IL-2Rγ chain, we analyzed whether IL-15 could skew monocyte differentiation into LCs. Monocytes cultured for 6 d with granulocyte/macrophage colony-stimulating factor (GM-CSF) and IL-15 differentiate into CD1a+HLA-DR+CD14-DCs (IL15-DCs). Agents such as lipopolysaccharide (LPS), tumor necrosis factor (TNF)α, and CD40L induce maturation of IL15-DCs to CD83+, DC-LAMP+ cells. IL15-DCs are potent antigen-presenting cells able to induce the primary (mixed lymphocyte reaction [MLR]) and secondary (recall responses to flu-matrix peptide) immune responses. As opposed to cultures made with GM-CSF/IL-4 (IL4-DCs), a proportion of IL15-DCs expresses LC markers: E-Cadherin, Langerin, and CC chemokine receptor (CCR)6. Accordingly, IL15-DCs, but not IL4-DCs, migrate in response to macrophage inflammatory protein (MIP)-3α/CCL20. However, IL15-DCs cannot be qualified as "genuine" Langerhans cells because, despite the presence of the 43-kD Langerin, they do not express bona fide Birbeck granules. Thus, our results demonstrate a novel pathway in monocyte differentiation into dendritic cells.

Idioma originalEnglish (US)
Páginas (desde-hasta)1013-1019
Número de páginas7
PublicaciónJournal of Experimental Medicine
Volumen194
N.º7
DOI
EstadoPublished - oct 1 2001
Publicado de forma externa

ASJC Scopus subject areas

  • General Medicine

Huella

Profundice en los temas de investigación de 'Interleukin 15 skews monocyte differentiation into dendritic cells with features of langerhans cells'. En conjunto forman una huella única.

Citar esto