Interfacially Engineered Pyridinium Pseudogemini Surfactants as Versatile and Efficient Supramolecular Delivery Systems for DNA, siRNA, and mRNA

Uttam Satyal, Bogdan Draghici, Lisa L. Dragic, Qiangnan Zhang, Kyle W. Norris, Muniswamy Madesh, Eugen Brailoiu, Marc A. Ilies

Producción científica: Articlerevisión exhaustiva

14 Citas (Scopus)

Resumen

This article presents the synthesis, self-assembly, and biological activity as transfection agents for pDNA, siRNA, and mRNA of novel pyridinium pseudogemini surfactants, interfacially engineered from the most efficient gemini surfactants and lipids generated in our amphiphile research program. Formulation of novel amphiphiles in water revealed supramolecular properties very similar to those of gemini surfactants, despite their lipidlike charge/mass ratio. This dual character was found also to enhance endosomal escape and significantly increase the transfection efficiency. We were also successful in identifying the parameters governing the efficient delivery of pDNA, siRNA, and mRNA, drawing valuable structure-activity and structure-property relationships for each nucleic acid type, and establishing DNA/siRNA/mRNA comparisons. Several supramolecular complexes identified in this study proved to be extremely efficient nucleic acid delivery systems, displaying excellent serum stability and tissue penetration in three-dimensional organoids.

Idioma originalEnglish (US)
Páginas (desde-hasta)29481-29495
Número de páginas15
PublicaciónACS Applied Materials and Interfaces
Volumen9
N.º35
DOI
EstadoPublished - sept 6 2017
Publicado de forma externa

ASJC Scopus subject areas

  • General Materials Science

Huella

Profundice en los temas de investigación de 'Interfacially Engineered Pyridinium Pseudogemini Surfactants as Versatile and Efficient Supramolecular Delivery Systems for DNA, siRNA, and mRNA'. En conjunto forman una huella única.

Citar esto