Insights into the mechanisms of flavoprotein oxidases from kinetic isotope effects

Paul F. Fitzpatrick

Resultado de la investigación: Review articlerevisión exhaustiva

20 Citas (Scopus)


Deuterium, solvent, and 15N kinetic isotope effects have been used to probe the mechanisms by which flavoproteins oxidize carbon-oxygen and carbon-nitrogen bonds in amines, hydroxy acids, and alcohols. For the amine oxidases D-amino acid oxidase, N-methyltryptophan oxidase, and tryptophan monooxygenase, D-serine, sarcosine, and alanine are slow substrates for which CH bond cleavage is fully rate limiting. Inverse isotope effects for each of 0.992-0.996 are consistent with a common mechanism involving hydride transfer from the uncharged amine. Computational analyses of possible mechanisms support this conclusion. Deuterium and solvent isotope effects with wild-type and mutant variants of the lactate dehydrogenase flavocytochrome b2 show that OH and CH bond cleavage are not concerted, but become so in the Y254F enzyme. This is consistent with a highly asynchronous reaction in which OH bond cleavage precedes hydride transfer. The results of Hammett analyses and solvent and deuterium isotope effects support a similar mechanism for alcohol oxidase.

Idioma originalEnglish (US)
Páginas (desde-hasta)1016-1025
Número de páginas10
PublicaciónJournal of Labelled Compounds and Radiopharmaceuticals
EstadoPublished - oct 30 2007
Publicado de forma externa

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Drug Discovery
  • Spectroscopy
  • Organic Chemistry


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