TY - JOUR
T1 - Injectable “Homing-Like” Bioactive Short-Fibers for Endometrial Repair and Efficient Live Births
AU - Cao, Yumeng
AU - Qi, Jia
AU - Wang, Juan
AU - Chen, Liang
AU - Wang, Yuan
AU - Long, Yijing
AU - Li, Boyu
AU - Lai, Junliang
AU - Yao, Yejie
AU - Meng, Yiwen
AU - Yu, Xiaohua
AU - Chen, Xiao Dong
AU - Ng, Lai Guan
AU - Li, Xinyu
AU - Lu, Yao
AU - Cheng, Xiaoyue
AU - Cui, Wenguo
AU - Sun, Yun
N1 - Publisher Copyright:
© 2024 The Authors. Advanced Science published by Wiley-VCH GmbH.
PY - 2024/5/28
Y1 - 2024/5/28
N2 - The prevalence of infertility caused by endometrial defects is steadily increasing, posing a significant challenge to women's reproductive health. In this study, injectable “homing-like” bioactive decellularized extracellular matrix short-fibers (DEFs) of porcine skin origin are innovatively designed for endometrial and fertility restoration. The DEFs can effectively bind to endometrial cells through noncovalent dipole interactions and release bioactive growth factors in situ. In vitro, the DEFs effectively attracted endometrial cells through the “homing-like” effect, enabling cell adhesion, spreading, and proliferation on their surface. Furthermore, the DEFs effectively facilitated the proliferation and angiogenesis of human primary endometrial stromal cells (HESCs) and human umbilical vein endothelial cells (HUVECs), and inhibited fibrosis of pretreated HESCs. In vivo, the DEFs significantly accelerated endometrial restoration, angiogenesis, and receptivity. Notably, the deposition of endometrial collagen decreased from 41.19 ± 2.16% to 14.15 ± 1.70% with DEFs treatment. Most importantly, in endometrium-injured rats, the use of DEFs increased the live birth rate from 30% to an impressive 90%, and the number and development of live births close to normal rats. The injectable “homing-like” bioactive DEFs system can achieve efficient live births and intrauterine injection of DEFs provides a new promising clinical strategy for endometrial factor infertility.
AB - The prevalence of infertility caused by endometrial defects is steadily increasing, posing a significant challenge to women's reproductive health. In this study, injectable “homing-like” bioactive decellularized extracellular matrix short-fibers (DEFs) of porcine skin origin are innovatively designed for endometrial and fertility restoration. The DEFs can effectively bind to endometrial cells through noncovalent dipole interactions and release bioactive growth factors in situ. In vitro, the DEFs effectively attracted endometrial cells through the “homing-like” effect, enabling cell adhesion, spreading, and proliferation on their surface. Furthermore, the DEFs effectively facilitated the proliferation and angiogenesis of human primary endometrial stromal cells (HESCs) and human umbilical vein endothelial cells (HUVECs), and inhibited fibrosis of pretreated HESCs. In vivo, the DEFs significantly accelerated endometrial restoration, angiogenesis, and receptivity. Notably, the deposition of endometrial collagen decreased from 41.19 ± 2.16% to 14.15 ± 1.70% with DEFs treatment. Most importantly, in endometrium-injured rats, the use of DEFs increased the live birth rate from 30% to an impressive 90%, and the number and development of live births close to normal rats. The injectable “homing-like” bioactive DEFs system can achieve efficient live births and intrauterine injection of DEFs provides a new promising clinical strategy for endometrial factor infertility.
KW - ECM short-fibers
KW - endometrial repair
KW - fertility enhancement
KW - intrauterine injection
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U2 - 10.1002/advs.202306507
DO - 10.1002/advs.202306507
M3 - Article
C2 - 38504456
AN - SCOPUS:85188070647
SN - 2198-3844
VL - 11
JO - Advanced Science
JF - Advanced Science
IS - 20
M1 - 2306507
ER -