@article{d98e2098db194547bd10f5937a0d8ee5,
title = "In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region",
abstract = "The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.",
author = "Mukouyama, \{Yoh Suke\} and Takahiko Hara and Xu, \{Ming Jiang\} and Kazuhiro Tamura and Donovan, \{Peter J.\} and Kim, \{Hee Jung\} and Hiroshi Kogo and Kohichiro Tsuji and Tatsutoshi Nakahata and Atsushi Miyajima",
note = "Funding Information: We thank T. Taga (Tokyo Medical and Dental University) for providing us with the anti-gp130 antibodies and S. Hayashi (Tottori University) for providing us with pairs of op/+ mice. Y. Mukouyama is supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan; a grant from New Energy and Industrial Technology Development Organization; a grant from Core Research for Evolutionary Science and Technology; a research grant from The Toray Research Foundation; and a research grant from Japan Science and Technology Corporation. This research was also sponsored in part by the National Cancer Institute, United States Department of Health and Human Services, under contract N01-CO-74101, to Advanced Bioscience Laboratories.",
year = "1998",
month = jan,
doi = "10.1016/S1074-7613(00)80463-X",
language = "English (US)",
volume = "8",
pages = "105--114",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "1",
}