In Situ Ligation: A Decade and a Half of Experience

Peter J. Hornsby, Vladimir V. Didenko

Resultado de la investigación: Chapter

19 Citas (Scopus)


The in situ ligation (ISL) methodology detects apoptotic cells by the presence of characteristic DNA double-strand breaks. A labeled double-stranded probe is ligated to the double-strand breaks in situ on tissue sections. Like the popular TUNEL assay, ISL detects cells in apoptosis based on the ongoing destruction of DNA by apoptotic nucleases. In comparison to TUNEL, it is more specific for apoptosis versus other causes of DNA damage, both repairable damage and necrosis. In the decade and a half since its introduction, ISL has been used in several hundred publications. Here we review the development of the method, its current status, and its uses and limitations.

Idioma originalEnglish (US)
Título de la publicación alojadaDNA Damage Detection In Situ, Ex Vivo, and In Vivo
Subtítulo de la publicación alojadaMethods and Protocols
EditorialHumana Press
Número de páginas15
ISBN (versión impresa)9781603274081
EstadoPublished - 2011

Serie de la publicación

NombreMethods in Molecular Biology
ISSN (versión impresa)1064-3745
ISSN (versión digital)1940-6029

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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