Hyperglycemia and xerostomia are key determinants of tooth decay in type 1 diabetic mice

Chih Ko Yeh, Stephen E. Harris, Sumathy Mohan, Diane Horn, Roberto Fajardo, Yong Hee Patricia Chun, James Jorgensen, Mary MacDougall, Sherry Abboud-Werner

Producción científica: Articlerevisión exhaustiva

37 Citas (Scopus)


Insulin-dependent type 1 diabetes mellitus (DM) and oral diseases are closely interrelated. Poor metabolic control in diabetics is associated with a high risk of gingivitis, periodontitis and tooth loss. Salivary flow declines in diabetics and patients suffer from xerostomia. Reduced saliva predisposes to enamel hypomineralization and caries formation; however, the mechanisms that initiate and lead to progression of tooth decay and periodontitis in type 1 DM have not been explored. To address this issue, we analyzed tooth morphology in Akita -/-mice that harbor a point mutation in the Ins2 insulin gene, which leads to progressive hyperglycemia. Mandibles from Akita-/-and wild-type littermates were analyzed by microCT, scanning EM and histology; teeth were examined for amelogenin (Amel) and ameloblastin (Ambn) expression. Mice were injected with pilocarpine to assess saliva production. As hyperglycemia may alter pulp repair, the effect of high glucose levels on the proliferation/ differentiation of cultured MD10-F2 pulp cells was also analyzed. Results showed that Akita -/-mice at 6 weeks of age showed chalky white incisors that correlated with marked hyperglycemia and impaired saliva production. MicroCT of Akita-/-teeth revealed excessive enamel wearing and hypomineralization; immunostaining for Amel and Ambn was decreased. A striking feature was invasion of dentinal tubules with Streptococcus mitis and microabcesses that originated in the coronal pulp and progressed to pulp necrosis and periapical periodontitis. High levels of glucose also inhibited MD10-F2 cell proliferation and differentiation. Our findings provide the first evidence that hyperglycemia in combination with reduced saliva in a model of type1 DM leads to decreased enamel mineralization/matrix proteins and predisposes to excessive wearing and decay. Importantly, hyperglycemia adversely affects enamel matrix proteins and pulp repair. Early detection and treatment of hyperglycemia and hyposalivation may provide a useful strategy for preventing the dental complications of diabetes and promoting oral health in this population.

Idioma originalEnglish (US)
Páginas (desde-hasta)868-882
Número de páginas15
PublicaciónLaboratory Investigation
EstadoPublished - jun 2012

ASJC Scopus subject areas

  • General Medicine


Profundice en los temas de investigación de 'Hyperglycemia and xerostomia are key determinants of tooth decay in type 1 diabetic mice'. En conjunto forman una huella única.

Citar esto