TY - JOUR
T1 - Human schistosomiasis mansoni
T2 - Intensity of infection differentially affects the production of interleukin-10, interferon-γ and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures
AU - Silveira, Alda M.S.
AU - Gazzinelli, Giovanni
AU - Alves-Oliveira, Lúcia F.
AU - Bethony, Jeffrey
AU - Gazzinelli, Andrea
AU - Carvalho-Queiroz, Claudia
AU - Alvarez, Maria Carolina B.
AU - Lima-Silva, Francisco C.
AU - Prata, Aluizio
AU - LoVerde, Philip T.
AU - Correa-Oliveira, Rodrigo
N1 - Funding Information:
This work was supported by grants from Programa de Apoio a Grupos de Excelência-PRONEX/CNPq/FINEP (Brazil), National Institutes of Health-NIH-ICIDR/Grant (AI45451-01, USA), Fundação de Amparo a Pesquisa do Estado de Minas Gerais-FAPEMIG (Brazil), Conselho Nacional de Desenvolvimento Cientı́fico e Tecnológico-CNPq (Brazil), and a UNDP World Bank/WHO Special Program for Research and Training in Tropical Diseases. J. Bethony is supported by an International Research Scientist Development Award (IRSDA) (1K01 TW00009-01) from the John C. Fogarty International Center, National Institutes of Health, USA.
PY - 2004/9
Y1 - 2004/9
N2 - The effect of the intensity of infection (eggs per gram faeces, epg) on the production of inteferon-γ (INF-γ), interleukin (IL)-10 and IL-13 by peripheral blood mononuclear cells (PBMCs) from individuals living in a Schistosoma mansoni -endemic area was evaluated. In vitro stimulation of PBMCs with soluble egg antigen (SEA) resulted in significantly higher secretion levels of IFN-γ in egg-negative individuals compared with those with an intensity of infection of more than 100 epg. In contrast, the egg-positive group produced significantly higher amounts of IL-10. Levels of IL-13 did not differ significantly between egg-positive and egg-negative groups. These findings suggest that IL-10 is an important cytokine in the control of the T helper cell (Th) type 1 responses during human S. mansoni infection, shifting the immune response from Th0 in egg-negative individuals from an endemic area to a Th2 polarization in chronic infected individuals.
AB - The effect of the intensity of infection (eggs per gram faeces, epg) on the production of inteferon-γ (INF-γ), interleukin (IL)-10 and IL-13 by peripheral blood mononuclear cells (PBMCs) from individuals living in a Schistosoma mansoni -endemic area was evaluated. In vitro stimulation of PBMCs with soluble egg antigen (SEA) resulted in significantly higher secretion levels of IFN-γ in egg-negative individuals compared with those with an intensity of infection of more than 100 epg. In contrast, the egg-positive group produced significantly higher amounts of IL-10. Levels of IL-13 did not differ significantly between egg-positive and egg-negative groups. These findings suggest that IL-10 is an important cytokine in the control of the T helper cell (Th) type 1 responses during human S. mansoni infection, shifting the immune response from Th0 in egg-negative individuals from an endemic area to a Th2 polarization in chronic infected individuals.
KW - Brazil
KW - Interferon-γ
KW - Interleukin-10
KW - Interleukin-13
KW - Schistosoma mansoni
KW - Schistosomiasis
UR - https://www.scopus.com/pages/publications/3342977308
UR - https://www.scopus.com/pages/publications/3342977308#tab=citedBy
U2 - 10.1016/j.trstmh.2003.11.009
DO - 10.1016/j.trstmh.2003.11.009
M3 - Article
C2 - 15251399
AN - SCOPUS:3342977308
SN - 0035-9203
VL - 98
SP - 514
EP - 519
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 9
ER -