TY - JOUR
T1 - Human pulp cells respond to calcitonin gene-related peptide in vitro
AU - Calland, John W.
AU - Harris, Stephen E.
AU - Carnes, David L.
N1 - Funding Information:
This research was supported in part by an Endodontic Resident Grant from the American Association of Endodontists Foundation.
PY - 1997
Y1 - 1997
N2 - Cell monolayers derived from human pulpal explants were passaged 3 to 4 times before characterization of the response of the cells to calcitonin gene-related peptide (CGRP). Northern blot analysis of mRNA revealed the presence of transcripts for bone morphogenetic protein-2 (BMP-2). Stimulation with CGRP produced a 1.8-fold increase in BMP-2 mRNA expression by the cells. Analysis of binding of CGRP to whole cells indicated that unlabeled human CGRP competed with labeled CGRP in a dose dependent fashion with a KD, estimated from the EC50, in the range of 5 × 10-8M. Binding of labeled CGRP was greatly reduced by the presence of 10-6M CGRP but was unaffected by the presence of 10-6M Human PTH(1-34) or 10-6M calcitonin. CGRP produced a 2.8-fold increase in cyclic AMP over basal levels, which was similar to the increase produced by PTH(1-34) (2.6 fold) but slightly more than the increase produced by calcitonin (1.9 fold). The pulp-derived cells displayed a high basal level of alkaline phosphatase enzyme activity, which was not altered by treatment with CGRP or either PTH{1-34) or 1,25(OH)2D3. Stimulation with 2.5 × 10-8M 1,25(OH)2D3 did produce a 7.6-fold increase in osteocalcin. These results indicate that pulp cells possess the cellular machinery to respond to CGRP and that stimulation of the production of BMP-2, a factor known to be associated with induction of dentin formation, is a component of the response.
AB - Cell monolayers derived from human pulpal explants were passaged 3 to 4 times before characterization of the response of the cells to calcitonin gene-related peptide (CGRP). Northern blot analysis of mRNA revealed the presence of transcripts for bone morphogenetic protein-2 (BMP-2). Stimulation with CGRP produced a 1.8-fold increase in BMP-2 mRNA expression by the cells. Analysis of binding of CGRP to whole cells indicated that unlabeled human CGRP competed with labeled CGRP in a dose dependent fashion with a KD, estimated from the EC50, in the range of 5 × 10-8M. Binding of labeled CGRP was greatly reduced by the presence of 10-6M CGRP but was unaffected by the presence of 10-6M Human PTH(1-34) or 10-6M calcitonin. CGRP produced a 2.8-fold increase in cyclic AMP over basal levels, which was similar to the increase produced by PTH(1-34) (2.6 fold) but slightly more than the increase produced by calcitonin (1.9 fold). The pulp-derived cells displayed a high basal level of alkaline phosphatase enzyme activity, which was not altered by treatment with CGRP or either PTH{1-34) or 1,25(OH)2D3. Stimulation with 2.5 × 10-8M 1,25(OH)2D3 did produce a 7.6-fold increase in osteocalcin. These results indicate that pulp cells possess the cellular machinery to respond to CGRP and that stimulation of the production of BMP-2, a factor known to be associated with induction of dentin formation, is a component of the response.
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U2 - 10.1016/S0099-2399(97)80306-8
DO - 10.1016/S0099-2399(97)80306-8
M3 - Article
C2 - 9587316
AN - SCOPUS:0031197181
SN - 0099-2399
VL - 23
SP - 485
EP - 489
JO - Journal of endodontics
JF - Journal of endodontics
IS - 8
ER -