High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

A. Ashok Kumar; Robert J. Kempton; Gregory M. Anstead; Elmer M. Price; James H. Freisheim

doi:10.1016/0003-2697(83)90362-7

High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

A. Ashok Kumar
, Robert J. Kempton
, Gregory M. Anstead
, Elmer M. Price
, James H. Freisheim

Producción científica: Article › revisión exhaustiva

14 Citas (Scopus)

Resumen

A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.

Idioma original	English (US)
Páginas (desde-hasta)	191-195
Número de páginas	5
Publicación	Analytical Biochemistry
Volumen	128
N.º	1
DOI	https://doi.org/10.1016/0003-2697(83)90362-7
Estado	Published - ene 1983
Publicado de forma externa	Sí

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/0003-2697(83)90362-7

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title = "High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives",

abstract = "A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.",

author = "Kumar, \{A. Ashok\} and Kempton, \{Robert J.\} and Anstead, \{Gregory M.\} and Price, \{Elmer M.\} and Freisheim, \{James H.\}",

note = "Funding Information: This work was supported in part by PHS Grant CA 11666, National Cancer Institute, DHHS (to J.H.F.), and by a grant from the Research Corporation (to R.J.K.). Grateful acknowledgement is made to the Albert and Louise Cooper Scholarship Fund for support of G.M.A. The authors thank Mr. Philip Smith and Mr. Larry Judge for their excellent technical assistance.",

year = "1983",

month = jan,

doi = "10.1016/0003-2697(83)90362-7",

language = "English (US)",

volume = "128",

pages = "191--195",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

AU - Kumar, A. Ashok

AU - Kempton, Robert J.

AU - Anstead, Gregory M.

AU - Price, Elmer M.

AU - Freisheim, James H.

N1 - Funding Information: This work was supported in part by PHS Grant CA 11666, National Cancer Institute, DHHS (to J.H.F.), and by a grant from the Research Corporation (to R.J.K.). Grateful acknowledgement is made to the Albert and Louise Cooper Scholarship Fund for support of G.M.A. The authors thank Mr. Philip Smith and Mr. Larry Judge for their excellent technical assistance.

PY - 1983/1

Y1 - 1983/1

N2 - A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.

AB - A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.

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U2 - 10.1016/0003-2697(83)90362-7

DO - 10.1016/0003-2697(83)90362-7

M3 - Article

C2 - 6405656

AN - SCOPUS:0020665356

SN - 0003-2697

VL - 128

SP - 191

EP - 195

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

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