TY - JOUR
T1 - High Energy Compound Stability During Experimental Brain Death
AU - Ferrera, R.
AU - Bopassa, J.
AU - Rodriguez, C.
AU - Baverel, G.
AU - Ovize, M.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/9
Y1 - 2006/9
N2 - The aim of this study was to examine the effect of sudden brain death (BD) on myocardial function and high energy phosphate (HEP) stores. BD was induced by cerebral vessel ligation in six swine (BD group) that were compared to six control swine. At the end of the BD period (3 hours), harvested hearts were stored at 4°C. Myocardial tissue HEP were assessed by: (i) 31P-NMR spectroscopy of left ventricle for phosphocreatine (PCr), adenosine triphosphate (ATP), inorganic phosphate (Pi) and intracellular pH (pHi), and by (ii) HPLC for ATP, ADP, and AMP levels in left ventricle biopsies. Brain death resulted in a instantaneous major increase in catecholamines (>50-fold, P < .001) and paradoxically a significant progressive decrease in the regional contractility of the left ventricle. After cardioplegia, no significant differences on HEP compounds (ATP/Pi, PCr/Pi, ATP, energetic index) or in pHi were observed between BD and control groups. These data suggest that early heart injury occuring during BD does not seem to be an ischemic phenomenon.
AB - The aim of this study was to examine the effect of sudden brain death (BD) on myocardial function and high energy phosphate (HEP) stores. BD was induced by cerebral vessel ligation in six swine (BD group) that were compared to six control swine. At the end of the BD period (3 hours), harvested hearts were stored at 4°C. Myocardial tissue HEP were assessed by: (i) 31P-NMR spectroscopy of left ventricle for phosphocreatine (PCr), adenosine triphosphate (ATP), inorganic phosphate (Pi) and intracellular pH (pHi), and by (ii) HPLC for ATP, ADP, and AMP levels in left ventricle biopsies. Brain death resulted in a instantaneous major increase in catecholamines (>50-fold, P < .001) and paradoxically a significant progressive decrease in the regional contractility of the left ventricle. After cardioplegia, no significant differences on HEP compounds (ATP/Pi, PCr/Pi, ATP, energetic index) or in pHi were observed between BD and control groups. These data suggest that early heart injury occuring during BD does not seem to be an ischemic phenomenon.
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U2 - 10.1016/j.transproceed.2006.06.123
DO - 10.1016/j.transproceed.2006.06.123
M3 - Article
C2 - 16980066
AN - SCOPUS:33846067172
SN - 0041-1345
VL - 38
SP - 2285
EP - 2286
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 7
ER -