High efficiency transduction of dendritic cells by adenoviral vectors targeted to DC-SIGN

Nikolay Korokhov, Tanja D. De Gruijl, Wayne A. Aldrich, Pierre L. Triozzi, Papia T. Banerjee, Stephen D. Gillies, Tyler J. Curiel, Joanne T. Douglas, Rik J. Scheper, David T. Curiel

Resultado de la investigación: Articlerevisión exhaustiva

34 Citas (Scopus)


Dendritic cells (DCs) are a central element in the development of antigen-specific immune responses. The lack of a specific and efficient technique for the in vivo delivery of antigens to DCs remains a major obstacle limiting a vaccine's ability to induce an effective immune response. The efficacy of adenoviral (Ad) vectors in this regard can be enhanced through alterations in vector tropism such that DC-targeted transduction is achieved. Here, the efficiency of DC transduction by Ad vectors retargeted to DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN) was studied and compared to that of Ad vectors retargeted through CD40. A comparable and significant enhancement of gene transfer to monocyte derived DCs (MDDCs) was accomplished by means of an Ad vector harboring the Fc-binding domain of Staphylococcus aureus protein A in combination with antibodies to DC-SIGN or to CD40 or with fused complexes of human Ig-Fc with their natural ligands, i.e., ICAM-3 or CD40L, respectively. Whereas CD40-targeted Ad transduction resulted in a more profound phenotypic DC maturation, DC-SIGN- and CD40-targeted Ad both induced similar levels of IL-12 secretion. These data demonstrate the usefulness of DC-SIGN as a DC-restricted targeting motif for Ad-mediated vaccination strategies.

Idioma originalEnglish (US)
Páginas (desde-hasta)289-294
Número de páginas6
PublicaciónCancer Biology and Therapy
EstadoPublished - mar 2005
Publicado de forma externa

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research


Profundice en los temas de investigación de 'High efficiency transduction of dendritic cells by adenoviral vectors targeted to DC-SIGN'. En conjunto forman una huella única.

Citar esto