Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples

J. Keith Killian, Robert L. Walker, Sven Bilke, Yidong Chen, Sean Davis, Robert Cornelison, William I. Smith, Paul S. Meltzer

Producción científica: Chapter

5 Citas (Scopus)

Resumen

New technologies allow for genome-scale measurement of DNA methylation. In an effort to increase the clinical utility of DNA methylation as a biomarker, we have adapted a commercial bisulfite epigenotyping assay for genome-wide methylation profiling in archival formalin-fixed paraffin-embedded pathology specimens. This chapter takes the reader step by step through a biomarker discovery experiment to identify phenotype-correlated DNA methylation signatures in routine pathology specimens.

Idioma originalEnglish (US)
Título de la publicación alojadaMolecular Profiling
Subtítulo de la publicación alojadaMethods and Protocols
EditoresVirginia Espina, Lance Liotta
Páginas107-118
Número de páginas12
DOI
EstadoPublished - 2012
Publicado de forma externa

Serie de la publicación

NombreMethods in Molecular Biology
Volumen823
ISSN (versión impresa)1064-3745

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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