Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

A. A. Korchagina; S. A. Shein; A. V. Leopol'D; N. E. Volgina; O. I. Gurina; I. P. Lazarenko; O. M. Antonova; V. P. Baklaushev; V. P. Chekhonin

doi:10.1007/s10517-014-2348-9

Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

A. A. Korchagina
, S. A. Shein
, A. V. Leopol'D
, N. E. Volgina
, O. I. Gurina
, I. P. Lazarenko
, O. M. Antonova
, V. P. Baklaushev
, V. P. Chekhonin

Producción científica: Article › revisión exhaustiva

6 Citas (Scopus)

Resumen

cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2_I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2_I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.

Idioma original	English (US)
Páginas (desde-hasta)	357-362
Número de páginas	6
Publicación	Bulletin of Experimental Biology and Medicine
Volumen	156
N.º	3
DOI	https://doi.org/10.1007/s10517-014-2348-9
Estado	Published - ene 2014
Publicado de forma externa	Sí

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1007/s10517-014-2348-9

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Korchagina, A. A., Shein, S. A., Leopol'D, A. V., Volgina, N. E., Gurina, O. I., Lazarenko, I. P., Antonova, O. M., Baklaushev, V. P., & Chekhonin, V. P. (2014). Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor. Bulletin of Experimental Biology and Medicine, 156(3), 357-362. https://doi.org/10.1007/s10517-014-2348-9

Korchagina, AA , Shein, SA, Leopol'D, AV, Volgina, NE, Gurina, OI, Lazarenko, IP, Antonova, OM, Baklaushev, VP & Chekhonin, VP 2014, 'Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor', Bulletin of Experimental Biology and Medicine, vol. 156, n.º 3, pp. 357-362. https://doi.org/10.1007/s10517-014-2348-9

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title = "Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor",

abstract = "cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.",

keywords = "competitive interaction, monoclonal antibodies, vascular endothelial growth factor",

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doi = "10.1007/s10517-014-2348-9",

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AU - Korchagina, A. A.

AU - Shein, S. A.

AU - Leopol'D, A. V.

AU - Volgina, N. E.

AU - Gurina, O. I.

AU - Lazarenko, I. P.

AU - Antonova, O. M.

AU - Baklaushev, V. P.

AU - Chekhonin, V. P.

PY - 2014/1

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N2 - cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.

AB - cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.

KW - competitive interaction

KW - monoclonal antibodies

KW - vascular endothelial growth factor

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JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

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Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

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