Generation of recombinant extracellular fragment of vascular endothelial growth factor receptor 2 and specific monoclonal antibodies to this receptor

A. A. Korchagina, S. A. Shein, A. V. Leopol'D, N. E. Volgina, O. I. Gurina, I. P. Lazarenko, O. M. Antonova, V. P. Baklaushev, V. P. Chekhonin

Producción científica: Articlerevisión exhaustiva

6 Citas (Scopus)

Resumen

cDNA extracellular Ig-like domains I-III of vascular endothelial growth factor receptor 2 (VEGFR2) was cloned in an expressing vector pET-32a. Western blotting showed immunochemical identity of recombinant VEGFR2I-III produced by prokaryotic expression system to the native receptor. BALB/c mice were immunized with VEGFR2I-III for obtaining specific antibodies to VEGFR2. Hybridomas producing monoclonal antibodies were selected by ELISA, Western blotting, and immunocytochemical assay. Thus, we obtained hybridoma producing monoclonal antibodies to VEGFR2 that selectively interact with both recombinant and native extracellular fragment of the receptor.

Idioma originalEnglish (US)
Páginas (desde-hasta)357-362
Número de páginas6
PublicaciónBulletin of Experimental Biology and Medicine
Volumen156
N.º3
DOI
EstadoPublished - ene 2014
Publicado de forma externa

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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