Gene Set Enrichment Analyses: Lessons learned from the heart failure phenotype

Vinicius Tragante, Johannes M.I.H. Gho, Janine F. Felix, Ramachandran S. Vasan, Nicholas L. Smith, Benjamin F. Voight, Colin Palmer, Pim Van Der Harst, Jason H. Moore, Folkert W. Asselbergs

Resultado de la investigación: Articlerevisión exhaustiva

4 Citas (Scopus)

Resumen

Background: Genetic studies for complex diseases have predominantly discovered main effects at individual loci, but have not focused on genomic and environmental contexts important for a phenotype. Gene Set Enrichment Analysis (GSEA) aims to address this by identifying sets of genes or biological pathways contributing to a phenotype, through gene-gene interactions or other mechanisms, which are not the focus of conventional association methods. Results: Approaches that utilize GSEA can now take input from array chips, either gene-centric or genome-wide, but are highly sensitive to study design, SNP selection and pruning strategies, SNP-to-gene mapping, and pathway definitions. Here, we present lessons learned from our experience with GSEA of heart failure, a particularly challenging phenotype due to its underlying heterogeneous etiology. Conclusions: This case study shows that proper data handling is essential to avoid false-positive results. Well-defined pipelines for quality control are needed to avoid reporting spurious results using GSEA.

Idioma originalEnglish (US)
Número de artículo18
PublicaciónBioData Mining
Volumen10
N.º1
DOI
EstadoPublished - 2017
Publicado de forma externa

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Computer Science Applications
  • Computational Mathematics
  • Computational Theory and Mathematics

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