GABAA receptor kinetics in the cerebellar nuclei: Evidence for detection of transmitter from distant release sites

Jason R. Pugh, Indira M. Raman

Resultado de la investigación: Articlerevisión exhaustiva

37 Citas (Scopus)


Neurons of the cerebellar nuclei receive GABAergic input from Purkinje cells. Purkinje boutons have several closely spaced presynaptic densities without GABA transporters, raising the possibility that neurotransmitter released by one presynaptic site diffuses to multiple postsynaptic sites. To test whether such local spillover may contribute to transmission, we studied gating of GABAA receptors at 31-33°C in cerebellar nuclear neurons acutely dissociated from mice. Currents were evoked by rapid application of long steps, brief pulses, and high-frequency trains of GABA to outside-out patches. Receptors desensitized and deactivated rapidly, and dose-response measurements estimated an EC50 of ∼30 μM. From these data, a kinetic scheme was developed that replicated the recorded currents. Next, we simulated diffusion of GABA in the synaptic cleft, constrained by previous electron microscopic data, and drove the kinetic GABAA receptor model with modeled concentration transients. Simulations predicted receptor occupancies of ∼100% directly opposite the release site and ∼50% at distant postsynaptic densities, such that receptors up to 700 nm from a release site opened on the timescale of the inhibitory postsynaptic currents before desensitizing. Further simulations of probabilistic release from multiple-site boutons suggested that local spillover-mediated transmission slows the onset and limits the extent of depression during high-frequency signaling.

Idioma originalEnglish (US)
Páginas (desde-hasta)1740-1754
Número de páginas15
PublicaciónBiophysical Journal
EstadoPublished - mar 2005
Publicado de forma externa

ASJC Scopus subject areas

  • Biophysics


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