TY - JOUR
T1 - Fusarium abutilonis and F. guadeloupense, two novel species in the Fusarium buharicum clade supported by multilocus molecular phylogenetic analyses
AU - O’Donnell, Kerry
AU - Gräfenhan, Tom
AU - Laraba, Imane
AU - Busman, Mark
AU - Proctor, Robert H.
AU - Kim, Hye Seon
AU - Wiederhold, Nathan P.
AU - Geiser, David M.
AU - Seifert, Keith A.
N1 - Funding Information:
This research was supported in part by the US Department of Agriculture, Agricultural Research Service National Program for Food Safety, National Science Foundation (NSF) grant DEB-1655980, Pennsylvania State Agricultural Experiment Station Project 4655, and CRTI project 04-0045RD. I.L. was supported in part by an appointment to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the US Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. All opinions expressed in this paper are the authors’ and do not necessarily reflect the policies and views of USDA, DOE, or ORAU/ORISE. The authors would like to thank Amy McGovern, Crystal Probyn, Ethan Roberts, and Deborah S. Shane for skilled assistance in various aspects of this study. T.G. and K.A.S. are grateful for contributions by Helgard I. Nirenberg in the early stages of this project. Thanks are due to the Canadian Collection of Fungal Cultures (DAOM, Ottawa) and the Institute for Epidemiology and Pathogen Diagnostics Culture Collection (Julius Kühn-Institute, Berlin) for providing cultures. Disclaimer : Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA). USDA is an equal opportunity provider and employer.
Publisher Copyright:
© 2022 Agricultural Research Service, United States Department of Agriculture.
PY - 2022
Y1 - 2022
N2 - This study was conducted to elucidate evolutionary relationships and species diversity within the Fusarium buharicum species complex (FBSC). We also evaluate the potential of these species to produce mycotoxins and other bioactive secondary metabolites. Maximum likelihood and maximum parsimony analyses of sequences from portions of four marker loci (ITS rDNA, TEF1, RPB1, and RPB2) and the combined 4495 bp data set support recognition of seven genealogically exclusive species within the FBSC. Two of the three newly discovered species are formally described as F. abutilonis and F. guadeloupense based on concordance of gene genealogies and morphological data. Fusarium abutilonis induces leaf, stem, and root lesions on several weedy Malvaceae (Abution theophrasti, Anoda cristata, Sida spinosa) and a fabaceous host (Senna obtusifolia) in North America and also was recovered from soil in New Caledonia. Fusarium abutilonis, together with its unnamed sister, Fusarium sp. ex common marsh mallow (Hibiscus moscheutos) from Washington state, and F. buharicum pathogenic to cotton and kenaf in Russia and Iran, respectively, were strongly supported as a clade of malvaceous pathogens. The four other species of the FBSC are not known to be phytopathogenic; however, F. guadeloupense was isolated from human blood in Texas and soil in Guadeloupe. The former isolate is unique because it represents the only known case of a fusarial infection disseminated hematogenously by a species lacking microconidia and the only documented fusariosis caused by a member of the FBSC. Whole genome sequence data and extracts of cracked maize kernel cultures were analyzed to assess the potential of FBSC isolates to produce mycotoxins, pigments, and phytohormones.
AB - This study was conducted to elucidate evolutionary relationships and species diversity within the Fusarium buharicum species complex (FBSC). We also evaluate the potential of these species to produce mycotoxins and other bioactive secondary metabolites. Maximum likelihood and maximum parsimony analyses of sequences from portions of four marker loci (ITS rDNA, TEF1, RPB1, and RPB2) and the combined 4495 bp data set support recognition of seven genealogically exclusive species within the FBSC. Two of the three newly discovered species are formally described as F. abutilonis and F. guadeloupense based on concordance of gene genealogies and morphological data. Fusarium abutilonis induces leaf, stem, and root lesions on several weedy Malvaceae (Abution theophrasti, Anoda cristata, Sida spinosa) and a fabaceous host (Senna obtusifolia) in North America and also was recovered from soil in New Caledonia. Fusarium abutilonis, together with its unnamed sister, Fusarium sp. ex common marsh mallow (Hibiscus moscheutos) from Washington state, and F. buharicum pathogenic to cotton and kenaf in Russia and Iran, respectively, were strongly supported as a clade of malvaceous pathogens. The four other species of the FBSC are not known to be phytopathogenic; however, F. guadeloupense was isolated from human blood in Texas and soil in Guadeloupe. The former isolate is unique because it represents the only known case of a fusarial infection disseminated hematogenously by a species lacking microconidia and the only documented fusariosis caused by a member of the FBSC. Whole genome sequence data and extracts of cracked maize kernel cultures were analyzed to assess the potential of FBSC isolates to produce mycotoxins, pigments, and phytohormones.
KW - 2 new taxa
KW - Chlamydospores
KW - RPB1
KW - RPB2
KW - TEF1
KW - equisetin
KW - genealogical concordance phylogenetic species recognition (GCPSR)
KW - morphology
KW - sporodochial conidia
KW - whole genome sequence
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U2 - 10.1080/00275514.2022.2071563
DO - 10.1080/00275514.2022.2071563
M3 - Article
C2 - 35679164
AN - SCOPUS:85131665473
SN - 0027-5514
VL - 114
SP - 682
EP - 696
JO - Mycologia
JF - Mycologia
IS - 4
ER -