TY - JOUR
T1 - Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system.
AU - Staruschenko, Alexander
AU - Booth, Rachell E.
AU - Pochynyuk, Oleh
AU - Stockand, James D.
AU - Tong, Qiusheng
PY - 2006
Y1 - 2006
N2 - Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.
AB - Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.
UR - https://www.scopus.com/pages/publications/39049190181
UR - https://www.scopus.com/pages/publications/39049190181#tab=citedBy
U2 - 10.1385/1-59745-095-2:3
DO - 10.1385/1-59745-095-2:3
M3 - Article
C2 - 16929934
AN - SCOPUS:39049190181
SN - 1064-3745
VL - 337
SP - 3
EP - 13
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -