Functional p85α gene is required for normal murine fetal erythropoiesis

Hannah Huddleston, Bailin Tan, Feng Chun Yang, Hilary White, Mary Jo Wenning, Attilio Orazi, Mervin C. Yoder, Reuben Kapur, David A. Ingram

Producción científica: Articlerevisión exhaustiva

27 Citas (Scopus)


In vitro studies suggest that activation of class IA phosphatidyiinositol 3 (PI-3) kinase is necessary for normal erythroid cell development. However, when class IA PI-3 kinase-deficient mice were generated by a targeted deletion of the p85α regulatory subunit, fetal erythropoiesis was reportedly unaffected. Given the discrepancies between these studies, we performed a more detailed in vivo analysis of class IA PI-3 kinase-deficient embryos. Day-14.5 p85α-/- embryos are pale with a marked reduction of mature erythrocytes in their peripheral blood. Further, the absolute number and frequency of both early (erythroid burst-forming unit [BFU-E]) and late erythroid progenitors (erythroid colony-forming unit [CFU-E]) are reduced in p85α-/- fetal livers compared with wild-type controls, which is associated with reduced proliferation. Taken together, these data establish an important role for p85α and class IA PI-3 kinase in regulating the development of both early and late erythroid progenitors in fetal liver.

Idioma originalEnglish (US)
Páginas (desde-hasta)142-145
Número de páginas4
EstadoPublished - jul 1 2003
Publicado de forma externa

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology


Profundice en los temas de investigación de 'Functional p85α gene is required for normal murine fetal erythropoiesis'. En conjunto forman una huella única.

Citar esto