@article{e6a00b12024944f38c67100fe42ff7ae,
title = "Functional attributes of the Saccharomyces cerevisiae meiotic recombinase Dmc1",
abstract = "The role of Dmc1 as a meiosis-specific general recombinase was first demonstrated in Saccharomyces cerevisiae. Progress in understanding the biochemical mechanism of ScDmc1 has been hampered by its tendency to form inactive aggregates. We have found that the inclusion of ATP during protein purification prevents Dmc1 aggregation. ScDmc1 so prepared is capable of forming D-loops and responsive to its accessory factors Rad54 and Rdh54. Negative staining electron microscopy and iterative helical real-space reconstruction revealed that the ScDmc1-ssDNA nucleoprotein filament harbors 6.5 protomers per turn with a pitch of ~106. {\AA}. The ScDmc1 purification procedure and companion molecular analyses should facilitate future studies on this recombinase.",
keywords = "Dmc1 recombinase, Homologous recombination, Meiosis",
author = "Valeria Busygina and Gaines, {William A.} and Yuanyuan Xu and Youngho Kwon and Williams, {Gareth J.} and Lin, {Sheng Wei} and Chang, {Hao Yen} and Peter Chi and Wang, {Hong Wei} and Patrick Sung",
note = "Funding Information: We are grateful to Douglas Bishop for the (His) 6 -ScDmc1 expression plasmid and to Edward Egelman for the IHRSR processing package used in the 3D reconstructions. Image acquisition and processing were conducted in the Yale CryoEM and High Performance Computational facilities. This study was supported by NIH grants RO1GM057814 , RO1ES007061 , and PO1CA092584 , NIH postdoctoral fellowship F32GM101808 , National Science Council of Taiwan grant NSC 100-2311-B-002-009 , National Taiwan University grants 102R7848 and 102R7560-6 , National Basic Research Program of China grant 2010CB912401 , and the National Center for Protein Sciences Beijing .",
year = "2013",
month = sep,
doi = "10.1016/j.dnarep.2013.05.004",
language = "English (US)",
volume = "12",
pages = "707--712",
journal = "DNA Repair",
issn = "1568-7864",
publisher = "Elsevier B.V.",
number = "9",
}