Förster resonance energy transfer-based imaging at the cell surface of live cells

Sonya M. Bierbower, Mark S. Shapiro

Resultado de la investigación: Chapter

3 Citas (Scopus)


Understanding the molecular mechanisms of protein-protein interactions at the cell surface of living cells is fundamental to identifying the nature of cellular processes. Here, we discuss how fluorescence-based approaches have been successfully developed to visualize protein-protein interactions in living cells. Förster resonance energy transfer (FRET) is unique in generating fluorescence signals between proteins that are highly spatially sensitive. Furthermore, total internal reflectance fluorescence (TIRF) microscopy combined with FRET is a robust technique used to assay protein/protein interactions and the functionality of proteins assembled at the cell surface membrane.

Idioma originalEnglish (US)
Título de la publicación alojadaIon Channels
Subtítulo de la publicación alojadaMethods and Protocols
EditoresNikita Gamper
Número de páginas8
EstadoPublished - 2013

Serie de la publicación

NombreMethods in Molecular Biology
ISSN (versión impresa)1064-3745

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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