Examination of the brain mitochondrial lipidome using shotgun lipidomics

Michael A. Kiebish, Xianlin Han, Thomas N. Seyfried

Producción científica: Articlerevisión exhaustiva

21 Citas (Scopus)

Resumen

Contamination from subcellular organelles and myelin has hindered attempts to characterize the lipidome of brain mitochondria. A high degree of mitochondrial purity is required for accurate measurements of the content and molecular species composition of mitochondrial lipids. We devised a discontinuous Ficoll and sucrose gradient procedure for the isolation and purification of brain mitochondria free from any detectable contamination. Shotgun lipidomics was used to analyze the lipid composition of the brain mitochondria. These procedures can be used to determine whether intrinsic lipid abnormalities underlie mitochondrial dysfunction associated with neurological and neurodegenerative diseases.

Idioma originalEnglish (US)
Páginas (desde-hasta)3-18
Número de páginas16
PublicaciónMethods in Molecular Biology
Volumen579
DOI
EstadoPublished - 2009
Publicado de forma externa

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Huella

Profundice en los temas de investigación de 'Examination of the brain mitochondrial lipidome using shotgun lipidomics'. En conjunto forman una huella única.

Citar esto