Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA)

Maria A. Lagarkova; Ekaterina P. Koroleva; Dmitry V. Kuprash; Veronica E. Boitchenko; Ulyana A. Kashkarova; Sergei A. Nedospasov; Yuriy V. Shebzukhov

doi:10.1016/S0165-2478(02)00209-2

Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA)

Maria A. Lagarkova, Ekaterina P. Koroleva, Dmitry V. Kuprash, Veronica E. Boitchenko, Ulyana A. Kashkarova, Sergei A. Nedospasov, Yuriy V. Shebzukhov

Producción científica: Article › revisión exhaustiva

27 Citas (Scopus)

Resumen

Screening of expression cDNA libraries derived from human neoplasms with autologous sera (SEREX) is an established method for defining antigens immunogenic in individual cancer patients. Although the majority of SEREX-derived cDNA clones encode autoantigens, some of them represent shared cancer antigens with cancer-related serological profiles. Routine evaluation of multiple SEREX-derived clones in serological assays using panels of allogeneic sera from cancer patients is an important step towards defining disease parameters of diagnostic and prognostic significance. Here we show how the seroreactivity of multiple SEREX-derived antigens can be simultaneously evaluated using a rapid semi-quantitative protocol of allogeneic screening, which we call SMARTA (serological mini-arrays of recombinant tumor antigens).

Idioma original	English (US)
Páginas (desde-hasta)	71-74
Número de páginas	4
Publicación	Immunology Letters
Volumen	85
N.º	1
DOI	https://doi.org/10.1016/S0165-2478(02)00209-2
Estado	Published - ene 2 2003
Publicado de forma externa	Sí

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0165-2478(02)00209-2

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title = "Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA)",

abstract = "Screening of expression cDNA libraries derived from human neoplasms with autologous sera (SEREX) is an established method for defining antigens immunogenic in individual cancer patients. Although the majority of SEREX-derived cDNA clones encode autoantigens, some of them represent shared cancer antigens with cancer-related serological profiles. Routine evaluation of multiple SEREX-derived clones in serological assays using panels of allogeneic sera from cancer patients is an important step towards defining disease parameters of diagnostic and prognostic significance. Here we show how the seroreactivity of multiple SEREX-derived antigens can be simultaneously evaluated using a rapid semi-quantitative protocol of allogeneic screening, which we call SMARTA (serological mini-arrays of recombinant tumor antigens).",

keywords = "Cancer antigens, Humoral response, Immunoscreening",

author = "Lagarkova, {Maria A.} and Koroleva, {Ekaterina P.} and Kuprash, {Dmitry V.} and Boitchenko, {Veronica E.} and Kashkarova, {Ulyana A.} and Nedospasov, {Sergei A.} and Shebzukhov, {Yuriy V.}",

note = "Funding Information: We thank Dr. L.J. Old for encouragement and continuing support, Dr. M.J. Scanlan for valuable discussions, Dr. A.A. Mescheryakov and Prof. M.R. Lichinitser (Oncological Scientific Center, Moscow) for providing clinical specimens, Dr. B.V. Radko for providing SEREX clone MO-OVA-42, Dr. S.V. Khlgatian, E. Pomerantseva, T. Kadachigova and R. Kazaryan for assistance. This work was supported by the Ludwig Institute for Cancer Research (through the GAP program of CRDF, Grant No. RBO-1226), Russian Foundation for Basic Research (Grant No. 02-04-48788), and by a fellowship from the Cancer Research Institute (M.A.L.).",

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doi = "10.1016/S0165-2478(02)00209-2",

language = "English (US)",

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T1 - Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA)

AU - Lagarkova, Maria A.

AU - Koroleva, Ekaterina P.

AU - Kuprash, Dmitry V.

AU - Boitchenko, Veronica E.

AU - Kashkarova, Ulyana A.

AU - Nedospasov, Sergei A.

AU - Shebzukhov, Yuriy V.

N1 - Funding Information: We thank Dr. L.J. Old for encouragement and continuing support, Dr. M.J. Scanlan for valuable discussions, Dr. A.A. Mescheryakov and Prof. M.R. Lichinitser (Oncological Scientific Center, Moscow) for providing clinical specimens, Dr. B.V. Radko for providing SEREX clone MO-OVA-42, Dr. S.V. Khlgatian, E. Pomerantseva, T. Kadachigova and R. Kazaryan for assistance. This work was supported by the Ludwig Institute for Cancer Research (through the GAP program of CRDF, Grant No. RBO-1226), Russian Foundation for Basic Research (Grant No. 02-04-48788), and by a fellowship from the Cancer Research Institute (M.A.L.).

PY - 2003/1/2

Y1 - 2003/1/2

N2 - Screening of expression cDNA libraries derived from human neoplasms with autologous sera (SEREX) is an established method for defining antigens immunogenic in individual cancer patients. Although the majority of SEREX-derived cDNA clones encode autoantigens, some of them represent shared cancer antigens with cancer-related serological profiles. Routine evaluation of multiple SEREX-derived clones in serological assays using panels of allogeneic sera from cancer patients is an important step towards defining disease parameters of diagnostic and prognostic significance. Here we show how the seroreactivity of multiple SEREX-derived antigens can be simultaneously evaluated using a rapid semi-quantitative protocol of allogeneic screening, which we call SMARTA (serological mini-arrays of recombinant tumor antigens).

AB - Screening of expression cDNA libraries derived from human neoplasms with autologous sera (SEREX) is an established method for defining antigens immunogenic in individual cancer patients. Although the majority of SEREX-derived cDNA clones encode autoantigens, some of them represent shared cancer antigens with cancer-related serological profiles. Routine evaluation of multiple SEREX-derived clones in serological assays using panels of allogeneic sera from cancer patients is an important step towards defining disease parameters of diagnostic and prognostic significance. Here we show how the seroreactivity of multiple SEREX-derived antigens can be simultaneously evaluated using a rapid semi-quantitative protocol of allogeneic screening, which we call SMARTA (serological mini-arrays of recombinant tumor antigens).

KW - Cancer antigens

KW - Humoral response

KW - Immunoscreening

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Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA)

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