TY - JOUR
T1 - Evaluation of antioxidant activity and characterization of phenolic constituents of Phyllanthus amarus root
AU - Maity, Soumya
AU - Chatterjee, Suchandra
AU - Variyar, Prasad Shekhar
AU - Sharma, Arun
AU - Adhikari, Soumyakanti
AU - Mazumder, Santasree
PY - 2013/4/10
Y1 - 2013/4/10
N2 - The antioxidant property of the 70% aqueous ethanol extract of Phyllanthus amarus roots and its ether-soluble, ethyl acetate-soluble, and aqueous fractions were investigated by various in vitro assays. The root extracts showed higher DPPH, hydroxyl, superoxide, and nitric oxide radical scavenging and reducing power activity. Among all the samples, the ethyl acetate-soluble fraction demonstrated highest radical scavenging activity and total phenolics content. Twenty-eight different phenolic compounds were identified by LCMS/MS analysis of the ethyl acetate-soluble fraction. The majority of the compounds were found to exist as their glycosides, and many of these were gallic acid derivatives. Free epicatechin and gallic acid were also identified in the ethyl acetate-soluble fraction. The present investigation suggested that P. amarus root is a potent antioxidant and can be used for the prevention of diseases related to oxidative stress.
AB - The antioxidant property of the 70% aqueous ethanol extract of Phyllanthus amarus roots and its ether-soluble, ethyl acetate-soluble, and aqueous fractions were investigated by various in vitro assays. The root extracts showed higher DPPH, hydroxyl, superoxide, and nitric oxide radical scavenging and reducing power activity. Among all the samples, the ethyl acetate-soluble fraction demonstrated highest radical scavenging activity and total phenolics content. Twenty-eight different phenolic compounds were identified by LCMS/MS analysis of the ethyl acetate-soluble fraction. The majority of the compounds were found to exist as their glycosides, and many of these were gallic acid derivatives. Free epicatechin and gallic acid were also identified in the ethyl acetate-soluble fraction. The present investigation suggested that P. amarus root is a potent antioxidant and can be used for the prevention of diseases related to oxidative stress.
KW - LCMS/MS analysis
KW - Phyllanthus amarus
KW - antioxidant activity
KW - gallic acid
KW - phenolic constituents
UR - http://www.scopus.com/inward/record.url?scp=84876146139&partnerID=8YFLogxK
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U2 - 10.1021/jf3046686
DO - 10.1021/jf3046686
M3 - Article
C2 - 23458355
AN - SCOPUS:84876146139
SN - 0021-8561
VL - 61
SP - 3443
EP - 3450
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 14
ER -