Estrogen effects on 5-HT1A receptors in hippocampal membranes from ovariectomized rats: functional and binding studies

William P. Clarke; Saul Maayani

doi:10.1016/0006-8993(90)90983-I

Estrogen effects on 5-HT_1A receptors in hippocampal membranes from ovariectomized rats: functional and binding studies

William P. Clarke, Saul Maayani

Producción científica: Article › revisión exhaustiva

55 Citas (Scopus)

Resumen

Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A₁ agonist) or Gpp(NH)p (to activate G_i). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT_1A binding sites labeled with [³H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT_1A-mediated responses in rat hippocampus.

Idioma original	English (US)
Páginas (desde-hasta)	287-291
Número de páginas	5
Publicación	Brain Research
Volumen	518
N.º	1-2
DOI	https://doi.org/10.1016/0006-8993(90)90983-I
Estado	Published - jun 4 1990
Publicado de forma externa	Sí

ASJC Scopus subject areas

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

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10.1016/0006-8993(90)90983-I

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title = "Estrogen effects on 5-HT1A receptors in hippocampal membranes from ovariectomized rats: functional and binding studies",

abstract = "Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A1 agonist) or Gpp(NH)p (to activate Gi). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT1A binding sites labeled with [3H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT1A-mediated responses in rat hippocampus.",

keywords = "8-Hydroxy-2-(di-n-propylamine)tetralin, Adenosine A, Adenylyl cyclase, Female, Guanosine 5′-(β,γ-imino)triphosphate, Guanosine 5′-triphosphate-binding protein, Serotonin",

author = "Clarke, {William P.} and Saul Maayani",

note = "Funding Information: This work was supported by United States Public Health Service Grants GM-34852 and DA-01875. W.P.C. is a Revson Fellow supported by an award from the Charles H. Revson Foundation. The authors would like to thank Kelly Ann Berg and Barbara Craddock-Royal for help with the adenylyl cyclase and binding assays.",

year = "1990",

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doi = "10.1016/0006-8993(90)90983-I",

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T2 - functional and binding studies

AU - Clarke, William P.

AU - Maayani, Saul

N1 - Funding Information: This work was supported by United States Public Health Service Grants GM-34852 and DA-01875. W.P.C. is a Revson Fellow supported by an award from the Charles H. Revson Foundation. The authors would like to thank Kelly Ann Berg and Barbara Craddock-Royal for help with the adenylyl cyclase and binding assays.

PY - 1990/6/4

Y1 - 1990/6/4

N2 - Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A1 agonist) or Gpp(NH)p (to activate Gi). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT1A binding sites labeled with [3H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT1A-mediated responses in rat hippocampus.

AB - Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A1 agonist) or Gpp(NH)p (to activate Gi). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT1A binding sites labeled with [3H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT1A-mediated responses in rat hippocampus.

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KW - Adenylyl cyclase

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KW - Guanosine 5′-triphosphate-binding protein

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